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Gautier EL, Shay T, Miller J, et al. Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nat Immunol. 13: 1118-1128

1] Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA. [2] The Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA. [3] Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
Nature Immunology (Impact Factor: 24.97). 09/2012; 13(11):1118-1128. DOI: 10.1038/ni.2419
Source: PubMed

ABSTRACT We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells.

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Article: Gautier EL, Shay T, Miller J, et al. Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nat Immunol. 13: 1118-1128

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    • "À and either MHC-II hi or MHC-II lo (R1 or R2, Figure 1A, respectively). Their macrophage identity was confirmed using the highly specific macrophage markers MerTK and CD64 (Figure 1B) (Gautier et al., 2012). "
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Questions & Answers about this publication

  • Alexander T Phythian-Adams added an answer in FACS:
    What markers can I use to differentiate mouse monocytes and dendritic cells in mouse lung samples?

    I want to FACS the lungs of mice. Can I use CD 11c to differentiate between dendritic and Monocytes in mice using FACS? Or what would be the best markers to use?

    Alexander T Phythian-Adams · The University of Manchester

    Good articles on this subject are:

    Gautier, E. L. et al. (2012). Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nature Immunology, 13(11), 1118–1128. doi:10.1038/ni.2419

    Plantinga, M. et al. (2013). Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen. Immunity, 38(2), 322–335. doi:10.1016/j.immuni.2012.10.016

    Summary:-

    Ly6C expression by monocyte derived cells is transient and CD64 is likely to be a better marker of monocyte origin. Conventional CD11b+ DCs should be CD64 negative whilst monocyte derived DCs are CD11b+CD64+. Note the use of MerTK and CD64 to better define macrophages in Gautier et al. 2012.