Biohydrogen Production from Cattle Wastewater by Enriched Anaerobic Mixed Consortia: Influence of Fermentation Temperature and pH

College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, PR China.
Journal of Bioscience and Bioengineering (Impact Factor: 1.88). 08/2008; 106(1):80-7. DOI: 10.1263/jbb.106.80
Source: PubMed


Experiments were conducted to select a natural mixed microflora seed source and investigate the effect of temperature and pH on fermentative hydrogen (H2) production from cattle wastewater by sewage sludge. Sewage sludge was shown to have higher cumulative H2 production than other inoculum collected from cow dung compost, chicken manure compost, and river sludge. Experimental results show that H2 production from cattle wastewater was significantly affected by both pH and temperature of the culture. The maximum H2 yield was obtained at pH 5.5. H2 yield and the ratio of butyrate/acetate (Bu/Ac) followed a similar production trend, suggesting that butyrate formation might favor H2 production. The optimal temperature for H2 production from cattle wastewater was 45 degrees C with peak values of H2 production (368 ml), hydrogen yield of 319 ml H2/g chemical oxygen demand (COD) consumed, and butyrate/acetate ratio of 1.43. Presence of ethanol and propionic acid indicated decreased hydrogen production; their concentrations were also affected by pH and temperature. A modified Gompertz model adequately described H2 production and bacterial growth.

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    05/2015; 2015:1-14. DOI:10.1155/2015/906094
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    • "Throughout the 5 day (static) fermentation, gas pressure was measured once a day using a digital manometer fitted with a syringe needle to determine biogas production, while gas samples (500 mL) were taken for hydrogen analysis. Subsequent fermentation experiments were conducted using the best pretreatment method and the conditions in Table 2. Since temperature and pH were reported to be critical parameters in the efficiency of biohydrogen production [26] [27], a factorial experiment was used to evaluate their impacts . Then, a study on biomass loading followed to evaluate high solids/high salts concentration using the optimum combination of temperature and initial pH determined previously . "
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    ABSTRACT: Duckweed harvested from a swine wastewater treatment system was evaluated for its potential as a fermentation feedstock for the production of biohydrogen. The effects of pretreatment and fermentation conditions on biohydrogen production were investigated in laboratory-scale batch experiments. The results showed that mild acidic thermal pretreatment (1% H2SO4 and 85 °C for 1 h) was more effective in improving the fermentability of duckweed than either thermal or mild alkaline thermal pretreatments. Fermentation of acid-pretreated duckweed resulted in a biohydrogen production of up to 75 mL H2 per g dry duckweed in 7 days (at a H2 concentration of 42%), which is comparable with other plant biomass. Overall, the results show that small aquatic plants such as duckweed can be promising substrate for biohydrogen production.
    International Journal of Hydrogen Energy 04/2015; 40(22). DOI:10.1016/j.ijhydene.2015.03.166 · 3.31 Impact Factor
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    • "However, there was little information on hydrogen production from OPEFB biomass hydrolyzate which is composed mainly of simple carbohydrate. Typically, the fermentation of biomass hydrolyzate, that uses wastewater sludge as inoculums, is influenced by parameters such as pH, temperature and substrate specificity [38]. The perception of the hydrogen production under different operational condition is extraordinarily narrowed, particularly with the use of mixed culture. "
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