The bacteriocin nisin, an effective agent for the treatment of staphylococcal mastitis during lactation.
ABSTRACT Eight women with clinical signs of staphylococcal mastitis were randomly divided in 2 groups. A solution of the bacteriocin nisin (6 microg/mL) was applied to the nipple and mammary areola of those assigned to the nisin group for 2 weeks, and a similar preparation devoid of nisin was applied to the control group. On day 0, staphylococcal counts in breast milk of the nisin and control groups were similar (5.04+/-0.19 and 4.88+/-0.21 log10 CFU/mL, respectively). However, on day 14, the mean in the nisin group (3.22+/-0.43 log10 CFU/mL) was statistically lower than that of the control group (5.01+/-0.21 log10 CFU/mL). No clinical signs of mastitis were observed among the women of the nisin group on day 14, whereas they persisted throughout the study in the women of the control group. In conclusion, nisin seems to be an efficient alternative to antibiotics for the treatment of staphylococcal mastitis.
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ABSTRACT: Although more than 200 bacteriocins have been isolated yet numerous antimicrobial compounds or BLIS are still to be fully characterized. The aim of the current study was to characterize novel significant aquatic Bacteriocins like inhibitory substance (BLIS). The two most significant and thermostable bacteriocinogenic aquatic strains were chosen out of previously isolated 10 strains for bio-physico-chemical characterization and identified as Bacillus coagulans and Bacillus spp. (ZP-108). Our findings showed that the bacteriocin was sensitive to trypsin as partially losing activity after enzyme treatment indicating the inhibitory substance contains the proteinaceous nature. The arbitrary units of Bacillus coagulans were 12500/mL having two weeks shelf life, while Bacillus spp. (ZP-108) having three weeks shelf life and 1250 arbitraty units/mL. Bacteriocinogenesis was optimum in Nutrient agar medium having 0.5% concentration of NaCl. Cell free neutralized supernatant (CFNS) of aquatic bacteriocinogenic strains exhibited activity within a wide pH range of 1-11 for Bacillus coagulans and 1-9 for Bacillus spp. (ZP-108). Bacillus spp. (ZP-108) was stable against organic solvents like methanol, acetone and chloroform while Bacillus coagulans against ethanol. Both strains were also resistant to the surfactant SDS. Even against other organic solvents and surfactants tested none of the strains lost more than 25% of their residual activity. Altogether the findings of the present study strongly suggest that both the thermostable bacteriocins having high titer and quite resistant to wide range of pH, organic solvents and surfactants can have a great potential for the broad based practical applications.International Journal of Data Mining and Bioinformatics 09/2014; 11:221-227. · 0.66 Impact Factor
Article: Safe Food additives. A review
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ABSTRACT: As studies uncover the breadth of microbes associated with human life, opportunities will emerge to manipulate and augment their functions in ways that improve health and longevity. From involvement in the complexities of reproduction and fetal/infant development, to delaying the onset of disease, and indeed countering many maladies, microbes offer hope for human well-being. Evidence is emerging to suggest that microbes may play a beneficial role in body sites traditionally viewed as being sterile. Although further evidence is required, we propose that much of medical dogma is about to change significantly through recognition and understanding of these hitherto unrecognized microbe–host interactions. A meeting of the International Scientific Association for Probiotics and Prebiotics held in Aberdeen, Scotland (June 2014), presented new views and challenged established concepts on the role of microbes in reproduction and health of the mother and infant. This article summarizes some of the main aspects of these discussions.American Journal Of Reproductive Immunology 09/2014; DOI:10.1111/aji.12319 · 3.32 Impact Factor