Attenuation of receptor-dependent and -independent vasoconstriction in the human radial artery

The Cardiothoracic Centre, Thomas Drive, Liverpool, UK.
European journal of cardio-thoracic surgery: official journal of the European Association for Cardio-thoracic Surgery (Impact Factor: 3.3). 09/2008; 34(4):839-44. DOI: 10.1016/j.ejcts.2008.06.016
Source: PubMed


Vasodilator strategies used to treat bypass grafts in the operating theatre, such as nitrates, phosphodiesterase inhibitors and calcium channel antagonists have a broad but short-lived effect against a variety of vasoconstrictor stimuli. Treatments that react irreversibly with proteins modulating vasoconstriction have the advantage that their effects can last well into the postoperative period. In addition systemic effects are avoided as the treatment is localised to the treated graft. This study investigated the use of two clinically applied drugs; fluphenazine (SKF7171A, HCl), an irreversible calmodulin antagonist and minoxidil sulphate, an irreversible potassium channel opener. Treatments were tested against receptor and non-receptor-mediated contraction in the human radial artery.
Isometric tension was measured in response to angiotensin II, KCl and vasopressin in 108 radial artery rings (taken from 31 patients undergoing coronary artery bypass grafting). Control responses were compared with rings pretreated with fluphenazine or minoxidil sulphate. Vasopressin responses were also compared in the presence of glyceryl trinitrate or the reversible Rho kinase inhibitor Y27632.
Fluphenazine pretreatment significantly suppressed vasoconstriction to all agonists tested. Maximal responses to angiotensin II, vasopressin and KCl were reduced by 42+/-19%, 35+/-8% and 48+/-15% respectively, without any measurable effect on the EC(50). Minoxidil sulphate showed no discernable effect. Vasopressin-induced contraction was also reduced by high levels of glyceryl trinitrate (220 microM; 50 microg/ml) or 10 microM Y27632.
The irreversible calmodulin antagonist fluphenazine has potential to be developed as an inhibitor of contraction in arterial graft vessels. The involvement of Rho kinase indicates that other vasoconstrictors and surgical stress can sensitize radial artery to vasopressin-induced contraction. Strategies targeting this pathway also have future potential.

21 Reads
  • Source
    • "Vasospasm of arterial conduits is an important cause of graft failure and morbidity in patients undergoing coronary artery surgery (He et al. 1995; Cable et al. 1998; Manabe and Sunamori 2006; Shackcloth et al. 2008). It is likely that the aetiology of graft vasospasm is multifactorial, including trauma at the time of surgery, disruption of the endothelium, and presence of vasoactive substances. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Radial artery (RA) vasospasm remains a potential cause of early graft failure after coronary artery bypass graft surgery, despite pretreatment with alpha-adrenergic or calcium channel blockers. Our aim was to investigate the mechanism of the vasorelaxant effects of Rho-kinase inhibitors (Y-27632 and fasudil) on the human RA. Segments were obtained from 30 patients undergoing coronary artery bypass graft and were divided into 3-4 mm vascular rings. The rings were stimulated with 10(-5) mol/L phenylephrine (PE) by using the isolated tissue bath technique and were relaxed with 10(-6) mol/L acetylcholine. Relaxation responses were recorded for Y-27632 (10(-9)-10(-4) mol/L), fasudil (10(-9)-10(-4) mol/L), and sodium nitroprusside (SNP) (10(-9)-10(-5) mol/L). Y-27632 and fasudil relaxation responses were repeated in either N(G)-nitro-L-arginine (L-NNA), which is a specific endothelial nitric oxide synthase inhibitor, or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), which is a guanylate cyclase inhibitor. SNP relaxation responses were repeated in 10(-8) mol/L Y-27632 and 10(-8) mol/L fasudil. Y-27632 and fasudil caused concentration-dependent vasorelaxation in RA rings precontracted with PE, and maximal relaxation (100%) was recorded at the highest concentration used (10(-4) mol/L). The vasorelaxant effects of Y-27632 and fasudil were significantly reduced in the presence of L-NNA and ODQ, and the pD2 values of Y-27632 and fasudil were not changed. The vasorelaxant effects of SNP were significantly increased in the presence of Y-27632 and fasudil, and the pD(2) values of SNP were not changed. These findings indicate that Y-27632 and fasudil caused concentration-dependent vasorelaxation in the RA rings. Because this effect was decreased in a dose-dependent manner by L-NNA and ODQ, the relaxant effects of Y-27632 and fasudil could be due to stimulation by nitric oxide that is being released. Rho-kinase inhibitors may have an important role in preventing vasospasm in arterial grafts used for coronary artery surgery.
    Canadian Journal of Physiology and Pharmacology 08/2009; 87(8):595-601. DOI:10.1139/y09-043 · 1.77 Impact Factor
  • Source
    • "The issue in question is whether this approach is performed at the expense of the radical resection required for the treatment of lung cancer. Access to the lung hilum through median sternotomy is easily carried out in cases of bilateral lung transplantation [2] or surgical treatment of pulmonary emphysema [3]. In cases of malignant disease this approach may compromise the extent of resection, mostly in terms of adequate mediastinal lymph node dissection, lead to a significant burden for the cardiac function and increase the risk of cancer cell's dissemination. "

    European journal of cardio-thoracic surgery: official journal of the European Association for Cardio-thoracic Surgery 05/2009; 35(6):1116-7. DOI:10.1016/j.ejcts.2009.02.006 · 3.30 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Neural networks and expert systems are now beginning to realise their potential in developing intelligent operational decision support systems. While neural networks are able to learn complex nonlinear functional relations between multiple inputs and outputs, there remains the important limitation that knowledge embedded in the neural network is opaque. This image of a black-box technology is a major factor influencing the acceptability of this approach because it does not improve the heuristic understanding of the domain problem. On the other hand, expert systems make use of logic rules to carry out heuristic reasoning. Knowledge used to reach a conclusion is transparent and can be displayed through HOW and WHY explanation facilities which are an integral part of well-founded expert systems. However, a critical problem with this approach is knowledge acquisition. This contribution introduces a fuzzy neural network to extract fuzzy rules automatically from numerical data. By changing fuzzy membership functions, three types of rules can be extracted, i.e. rules with and without fuzzy membership values and neuro-expert systems which overcome the problems described above.
    Computers & Chemical Engineering 05/1997; 21. DOI:10.1016/S0098-1354(97)87578-3 · 2.78 Impact Factor
Show more


21 Reads