Biochemical and histochemical studies on non-specific phosphomonoesterases of swine kidney worm Stephanurus dentatus (Diesing, 1839).
ABSTRACT Biochemical and histochemical studies have been made on non-specific acid and alkaline phosphomonoesterases of S. dentatus. The two forms of acid phosphomonoesterases have been found active at pH 4.0 and 6.0. The pH optima for the two forms of aklaline phosphomonoesterases lie at 8.0 and 10.0. Studies on the distribution of acid and alkaline phosphomonoesterases in various tissues have revealed an abundance of acid phosphomonoesterase in various parts of the alimentary canal and various organs of the reproductive system. The excretory ducts show alkaline phosphomonoesterase activity only.
- Parasitology 05/1972; 64(2):347-53. · 2.36 Impact Factor
- Biochemical Journal 08/1962; 84:192-5. · 4.65 Impact Factor
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ABSTRACT: 1.1. Hatched second-stage Ascaris larvae have been shown to contain fructose-1,6-diphosphatase and phosphoenolpyruvate carboxykinase, both of which are required for glyconeogenesis which, in turn, is required for the proposed pathway for larval metabolism.2.2. In contrast to mammalian skeletal and smooth muscle, adult Ascaris muscle possesses both fructose-1,6-diphosphatase and phosphoenolpyruvate carboxykinase activities. In addition, NaH14CO3 and succinate-2,3-14C are incorporated into glycogen when incubated with Ascaris muscle strips.3.3. As is the case with the corresponding enzyme from mammalian striated muscle, fructose-1,6-diphophatase preparations from both Ascaris muscle and larvae require Mg2+ and EDTA for optimal activity, and are inhibited by AMP as well as by high substrate concentrations.4.4. Attempts to demonstrate phosphoenolpyruvate carboxykinase activity in the smooth muscle, taenia coli, were negative.Comparative Biochemistry and Physiology 08/1967; 22(1):15-28.