Article

Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein.

Division of Nephrology, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA. Electronic address: .
FEBS letters (Impact Factor: 3.54). 09/2012; DOI: 10.1016/j.febslet.2012.09.015
Source: PubMed

ABSTRACT Aminoacylase 3 (AA3) mediates deacetylation of N-acetyl aromatic amino acids and mercapturic acids. Deacetylation of mercapturic acids of exo- and endobiotics are likely involved in their toxicity. AA3 is predominantly expressed in kidney, and to a lesser extent in liver, brain, and blood. AA3 has been recently reported to interact with the hepatitis C virus core protein (HCVCP) in the yeast two-hybrid system. Here we demonstrate that AA3 directly binds to HCVCP (K(d) ∼10μM) that may by implicated in HCV pathogenesis. AA3 also revealed a weak endopeptidase activity towards the N-terminus of HCVCP. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: AA3cleavesHCVCP by protease assay (View interaction). AA3cleavesAA3 by protease assay (View interaction). AA3binds to HCVCP by surface plasmon resonance (View Interaction:1,2,3,4,5).

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