Genome sequences of a novel HIV −1 CRF53_01B identified in Malaysia

Centre of Excellence for Research in AIDS (CERiA), Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Journal of Virology (Impact Factor: 4.44). 10/2012; 86(20):11398-9. DOI: 10.1128/JVI.01932-12
Source: PubMed


A novel HIV-1 genotype designated CRF53_01B was recently characterized from three epidemiologically unrelated persons in Malaysia.
Here we announced three recently isolated full-length genomes of CRF53_01B, which is likely to be phylogenetically linked
to CRF33_01B, circulating widely in Southeast Asia. The genome sequences may contribute to HIV-1 molecular surveillance and
future vaccine development in the region.

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Available from: Haider Al-Darraji,
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    • "Recombination breakpoint analysis revealed that these six NFLG sequences shared identical recombinant structures in which two subtype B regions (nucleotide position 2570–2718 in the pol region and 6149–8243 nt in the region relative to the HXB2 genome) were located in a CRF01_AE backbone (Figure 3). The recombinant structure is distinct from any other known CRFs comprising CRF01_AE and subtype B, including CRF15_01B [22], CRF33_01B [23], CRF34_01B [24], CRF48_01B [25], CRF51_01B [3], CRF52_01B [4], CRF53_01B [5], CRF54_01B [6], and CRF55 01B [7]. Subregion tree analyses further confirmed the parental origins of each region of the recombinant genome as follows (Figure 3C): Region I (HXB2: 790–2569) = CRF01_AE; Region II (HXB2: 2570–2718) = B; Region III (HXB2: 2719–6148) = CRF01_AE; Region IV (HXB2: 6149–8243) = B; Region V (HXB2: 8244–9600) = CRF01_AE. "
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    ABSTRACT: The HIV-1 epidemic among men-who-have-sex-with-men (MSM) continues to expand in China. A large-scale national survey we conducted on HIV-1 strains among MSM in 11 provinces in China from 2008 to 2013 (n = 920) identified a novel transmission cluster consisting of six strains (0.7%) that belonged to a new circulating recombinant form (designated CRF59_01B). CRF59_01B contains two subtype B segments of U.S.-European origin (in the pol and vpu-env regions) in a CRF01_AE backbone. CRF59_01B is the second CRF (after CRF55_01B) circulating primarily among MSM in China. CRF59_01B occurs at a low frequency (less than 1%), but it was detected in four different provinces/regions in China: Liaoning (northeast China) (n = 3); Hunan (central China) (n = 1); Guangdong (south China) (n = 1); Yunnan (southwest China) (n = 1). One additional recombinant strain was detected in a heterosexual individual in Liaoning province but is not the focus of this paper. Bayesian molecular clock analyses indicate that CRF59_01B emerged as a result of recombination between CRF01_AE and subtype B around the year 2001. The emergence of multiple forms of recombinants and CRFs reflects the ever-increasing contribution of homosexual transmission in China's HIV epidemic and indicates an active HIV transmission network among MSM in China.
    PLoS ONE 06/2014; 9(6):e99693. DOI:10.1371/journal.pone.0099693 · 3.23 Impact Factor
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    ABSTRACT: We report here a novel HIV-1 circulating recombinant form (CRF55_01B) composed of CRF01_AE and subtype B, with four recombination breakpoints in the pol gene. CRF55_01B was identified from three epidemiologically unlinked men having sex with men (MSM) in China, suggesting the ongoing generation of recombinants involving CRF01_AE and subtype B lineages among MSM in China.
    Genome Announcements 01/2013; 1(1). DOI:10.1128/genomeA.00050-12
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    ABSTRACT: We report here a novel HIV-1 circulating recombinant form (CRF) (CRF59_01B) comprised of CRF01_AE and subtype B, with two recombination breakpoints in the pol and vpu-env regions, respectively. CRF59_01B was identified from three epidemiologically unlinked men who have sex with men (MSM) in northeast China. This represents the second CRF identified in the MSM population in China.
    Genome Announcements 05/2013; 1(3). DOI:10.1128/genomeA.00315-13
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