Reduction of Listeria monocytogenes Biofilms on Stainless Steel and Polystyrene Surfaces by Essential Oils

Department of Food Science, Nutrition and Health Promotion, Mississippi State University, Mississippi State, Mississippi 39762, USA.
Journal of food protection (Impact Factor: 1.8). 07/2012; 75(7):1332-7. DOI: 10.4315/0362-028X.JFP-11-517
Source: PubMed

ABSTRACT Plant-derived essential oils were tested for their ability to eliminate biofilms of Listeria monocytogenes on polystyrene and stainless steel surfaces. Various concentrations of essential oils were tested with different contact times on biofilms of various ages. Preliminarily screening of nine essential oils and related phenolic compounds in a disk diffusion assay revealed that thyme oil, oregano oil, and carvacrol had the highest antimicrobial activity. Further screening of these three compounds against 21 L. monocytogenes strains representing all 13 serotypes indicated some strain-specific variations in antimicrobial activity. For 1-day-old biofilms of mixed L. monocytogenes strains produced at 22°C on polystyrene microtiter plates, only 0.1% concentrations of thyme oil, oregano oil, and carvacrol were needed to eliminate 7 log CFU per well. On the stainless steel coupons, a 0.5% concentration of these compounds was adequate to completely eliminate 4-day-old biofilms at 7 log CFU per coupon. Our findings indicate that these compounds are potential candidates for elimination of L. monocytogenes biofilms on stainless steel and polystyrene surfaces.

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Available from: Monil A Desai, Apr 10, 2014
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    • "To reduce the incidence of listeriosis, various new strategies have been proposed including natural antimicrobials as alternative or " natural sanitizers " instead of chemicals, e.g. essential oils (Desai et al., 2012), nisin (Guerra et al., 2005) and reuterin alone or in combination with nisin (El-Ziney and Jakobsen, 2009). Enterocin AS-48 is a cyclic antimicrobial peptide produced by Enterococcus faecalis and Enterococcus faecium strains (Maqueda et al., 2004; Abriouel et al., 2010). "
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