Culture of Azolla filiculoides in artificial conditions

Plant Biosystems (Impact Factor: 1.91). 11/2009; 2009(143):431–434. DOI: 10.1080/11263500903172110

ABSTRACT Azolla filiculoides showed a planar development in four culture media, but with overlapping of sporophytes after 28 days, and curled roots in all cases except for IRRI2. The difference in biomass between the media IRRI2 and IRRI1-Fe10x was statistically significant at Days 14, 21 and 28 by ANOVA. Medium IRRI2 gave the highest duplication time.

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Available from: Ana L. Pereira, Jul 02, 2015
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    ABSTRACT: Previous reports indicate the use of the aquatic fern Azolla as medicinal plant in New Zealand and Tanzania against sore throat and cough, respectively. Therefore, the aims were to evaluate the bioactivity of Azolla organic and aqueous extracts against bacteria and yeasts. Organic (dichloromethane:methanol) and aqueous extracts obtained from six Azolla species were tested against bacterial pathogenic and non-pathogenic strains (four Gram-positive and three Gram-negative) and pathogenic fungi (three clinical isolates of Candida albicans and one of C. glabrata), using the agar diffusion method and the broth microdilution assay. The results showed that organic extracts of A. caroliniana and A. rubra and of A. filiculoides inhibited the growth of B. subtilis ATCC 6633 whereas those of A. caroliniana and A. microphylla inhibited the growth of S. aureus ATCC 25923. The MICs were higher than 4 mg/ml for A. caroliniana, A. microphylla and A. rubra and higher than 3.25 mg/ml for A. filiculoides. Aqueous extracts of A. filiculoides, A. caroliniana, A. microphylla, A. rubra and A. pinnata var. pinnata induce a small inhibition zone (1 mm) in C. albicans ATCC 10231 with a MIC higher than 12.5 mg/ml. In conclusion, organic and aqueous extracts of some Azolla species show potential for use against infections caused by Gram-positive bacteria and C. albicans, respectively.
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