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Quantitative Analysis of Total Phenolic, Flavonoids and Tannin Contents in Acetone and n-hexane Extracts of Ageratum conyzoides

ABSTRACT Ageratum conyzoides an annual herbaceous plant is found in tropical and sub-tropical regions. Present study was aimed to quantitative analysis of total phenolic, total flavonoids, tannin content present in different extracts. Total phenolic content is significantly high (P<0.05) in n-hexane extract 34.62 ± 0.94 mg of GAE/gm of extract as compared to acetone extract 25.70 ± 2.00 mg of GAE/gm of extract. Similarly significant high (P<0.05) concentrations of flavonoids (1172.55 ± 17.69 mg Quercetin/gm dried extract) and non-tannin content (12.30 ± 0.97 mg of GAE/gm of extract) have been observed in n-hexane extract as compared to acetone extract. Thus study suggested that n-hexane extract of plant have more potential in scavenging the free radicals/ROS as compared to acetone extract.

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    ABSTRACT: Ageratum conyzoides L. (Compositae) has been used in various parts of the world for the treatment of various diseases based on their age-old use in folklore system. Present study was aimed to investigate the hepatoprotective activity of acetone and n-hexane extracts of Ageratum conyzoides in wistar rats following acetaminophen (APAP) induced hepatotoxicity. Single high dose exposure of APAP significantly (p<0.05) increased in ALT, AST, and GGT activity and levels of BUN, CR, unconjugated bilirubin and A/G ratio, whereas activity of LDH-P, total protein, albumin, globulin and conjugated bilirubin were significantly (p<0.05) reduced as compared to control. Pre-exposure with acetone and n-hexane extracts of A. conyzoides restore the values of ALT, GGT, LDH-P, albumin, unconjugated and conjugated bilirubin as compared to control whereas the AST, globulin, A/G ratio, BUN and CR levels are not restored by administration of plant extracts. It is evident from observations that acetone and n-hexane extracts of A. conyzoides was able to restore the levels of SGPT, SGOT, LDH and bilirubin as an indication of the stabilization of plasma membrane as well as repair of hepatic tissue damages caused by APAP.
    Journal of Applied Pharmaceutical Science 04/2013; 3(4):23-27. DOI:10.7324/JAPS.2013.34.S4
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    ABSTRACT: Erythrocytes are more vulnerable to oxidative stress among eukaryotic cells because it contains no nucleus and cytoplasmic organelles with high haemoglobin concentration. Present study was aimed to investigate the antioxidant status of the erythrocytes during hepatotoxicity and protective effects of extracts of Ageratum conyzoides in wistar rats. Antioxidant parameters like total thiols, GSH levels, activities of G6PD, GST and SOD and oxidative damage indicator MDA level have been determined to access the antioxidant status during hepatotoxicity induced by acetaminophen (APAP) and its protection by acetone and n-hexane extracts. Significant (p<0.05) increased in MDA, GST activity and significantly (p<0.05) decreased GSH, total thiols and activities of SOD & G6PD was observed in APAP exposed group as compared to control. The pre-treatment with acetone and n-hexane extracts of A. conyzoides fallowed by APAP exposure significantly (p<0.05) increased activities of G6PD and significantly (p<0.05) reduced activity of GST and MDA levels as compare to APAP exposed group. Protective effects of extracts of A. conyzoides may be due to presence of high concentration of phenolic; flavonoids and beta-caryophyllene contents, these agents have been reported to reduce free radical generation, pro-inflammatory proteins production and induces phase II biotransformation reactions. Observation suggested that APAP exposure at high dose induces oxidative stress in erythrocytes and n-hexane extract is better in combating the oxidative stress of erythrocytes as compare to acetone extract of A. conyzoides.
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    ABSTRACT: Background and objectives Drug induced hepatotoxicity is a major health issue concern by drug regulatory agencies, pharmaceutical industry and health care professionals. Present study was aimed to investigate the hepatoprotective mechanisms of n-hexane and acetone extract of Ageratum conyzoides on oxidative damage induced by acetaminophen (APAP) in Wistar rats. Materials and methods 42 Wistar rats were randomly divided into seven groups. Group I and II receive distilled water, CMC respectively. Group III was fed with standard drug silymarin, group IV and V received plant extracts only whereas group VI and VII received pretreatment with acetone and n-hexane extracts for seven days respectively and APAP was administered on the 5th day. Samples were collected on 48 h of post APAP administration and analysis was done using standard protocols. Results Significant (p < 0.05) increased in MDA, G6PD and GST and significant (p < 0.05) decrease in SOD, GSH, total thiols in liver tissue was observed in APAP exposed group as compared to control. The pre-exposure of acetone and n-hexane extracts of A. conyzoides fallowed by APAP exposure significantly (p < 0.05) reduce activities of G6PD, GST and MDA levels as compare to APAP exposed group, whereas total thiols and GSH levels are restored only in n-hexane extract of A. conyzoides. Conclusion Observations of present study suggested that pre-exposure of n-hexane extract of A. conyzoides restore the levels of total thiols, GSH and GST activity which may be responsible for reducing oxidative damage induced by APAP administration.
    10/2013; 3(2). DOI:10.1016/j.fra.2013.05.009