Assembly of DNA Curtains Using Hydrogen Silsesquioxane As a Barrier to Lipid Diffusion

Department of Applied Physics and Applied Mathematics, Center for Electron Transport in Molecular Nanostructures, NanoMedicine Center for Mechanical Biology, Columbia University , 1020 Schapiro CEPSR, 530 West 120th Street, New York, New York 10027, United States.
Analytical Chemistry (Impact Factor: 5.64). 09/2012; 84(18):7613-7. DOI: 10.1021/ac302149g
Source: PubMed


We have established a single-molecule imaging experimental platform called "DNA curtains" in which DNA molecules tethered to a lipid bilayer are organized into patterns at nanofabricated metallic barriers on the surface of a microfluidic sample chamber. This technology has wide applications for real-time single-molecule imaging of protein-nucleic acid interactions. Here, we demonstrate that DNA curtains can also be made from hydrogen silsesquioxane (HSQ). HSQ offers important advantages over metallic barriers because it can be lithographically patterned directly onto fused silica slides without any requirement for further processing steps, thereby offering the potential for rapid prototype development and/or scale up for manufacturing.

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