Antimicrobial and Enzyme Activity of Endophytic Fungi Isolated from Tulsi


ABSTRACT A study was carried out to isolate endophytic fungi from Ocimum species (Tulsi) and to assess their antimicrobial activity against Pseudomonas aeroginosa, Mycobacterium smegmatis, Salmonella typhimurium, Candida albicans and Penicillium chrysogenum and production of enzymes such as amylase, protease and tyrosinase. In this study forty endophytic fungi were isolated from leaves and branches of Tulsi. Out of forty endophytic fungal isolates, six isolates showed the inhibition activity against test microorganisms done by dual culture method. The isolate P14T1 exhibited a high antimicrobial activity against Candida albicans (22mm). The crude extract of P13T5 isolate showed highest zone of inhibition against Pseudomonas aeroginosa (21mm) by well and disc diffusion method. 50% of fungal isolates found positive for amylase and protease enzymes production and 27.5% showed positive for tyrosinase. The results of the study suggest that endophytic fungi associated with Tulsi are potential agents for antimicrobial activity and production of enzyme.

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    ABSTRACT: Five different endophytic fungi was isolated and identified from the various parts of Viscum album, protein isolated from the endophytic fungi Alternaria sp and Viscum album was identified as lectin based on haemagglutination assay, isolated lectin was blood group specific lectin agglutinating A+ve erythrocytes. SDSPAGE showed the molecular weight of 64 kDa protein was observed in endophytic Alternaria sp similar to Viscum album, PAS staining of NATIVE PAGE confirms the presence of lectin, MALDI-TOF-MS analysis of endophytic fungal lectin and MASCOT peptide mass finger printing reported that endophytic fungal lectin belongs to antiviral protein and ribosome inactivating protein family, observed peptide sequence K.NGALPKPLELK, identified as a conserved sequence pattern in the ribosome inactivating protein (RIPL1_PHYDI) of Phytolacca dioica (Bella sombra) tree and antiviral protein(RIPS_PHYAM) of Phytolacca americana (American pokeweed), this confirms that endophytic fungi Alternaria sp capable of producing lectin having antiviral and anticancer activity. Endophytic fungal lectin showed in vitro antibacterial activity on human pathogenic bacteria, maximum inhibition of 9 mm in Pseudomonas sp, 11 mm inhibition in Salmonella sp was observed from endophytic lectin extract.
    Drug Discovery Today 08/2014; 6(2):105-112. · 6.55 Impact Factor


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Jun 3, 2014