Quantitative Determination of Sugar Cane Sucrose by Multidimensional Statistical Analysis of their Mid-Infrared Attenuated Total Reflectance Spectra*

Applied Spectroscopy (Impact Factor: 1.94). 01/1991; 45(2):166-172. DOI: 10.1366/0003702914337470

ABSTRACT A fast and accurate method for determining the sucrose content of sugar cane juice has been developed. The application of principal component regression (PCR) has been proposed for the development of a prediction equation of sucrose content by mid-infrared spectroscopy. An attenuated total reflectance (ÀTR) cell is used in place of the more familiar hans-mission cell. PCR involves two steps: (1) the creation of new synthetic variables by principal component analysis (PCA) of spectral data, and (2) multiple linear regression (MLR) with these new variables. Results obtained by this procedure have been compared with those obtained by the conventional application of polarization.

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