Article

Probing Translation Initiation through Ligand Binding to the 5' mRNA Coding Region.

Department of Biotechnology, Yonsei University, Shinchon-Dong 134, Seodaemun-Gu, Seoul 120-749 (South Korea).
ChemBioChem (impact factor: 3.94). 08/2012; 13(14):2048-51. DOI:10.1002/cbic.201200432 pp.2048-51
Source: PubMed

ABSTRACT Secondary structure matters: We have constructed artificial intragenic riboswitches to probe ribosome accessibility to the 5' mRNA coding region at three-base resolution in Escherichia coli. We show that only mRNA folding stability in the +1 to +15 nt region affects the translation process.

0 0
 · 
0 Bookmarks
 · 
24 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

5' mRNA coding region
 
mRNA folding stability
 
probe ribosome accessibility
 
Secondary structure matters
 

Joon-Jung Jo