Genome Sequence of an Unusual Human G10P Rotavirus Detected
Yuki Matsushima,aEtsuko Nakajima,aTuan Anh Nguyen,bHideaki Shimizu,aAtsuko Kano,aYoko Ishimaru,aTung Gia Phan,cand
Division of Virology, Kawasaki City Institute of Public Health, Oshima, Kawasaki-ku, Kawasaki-shi, Kanagawa, Japana; Department of Gastroenterology, Children’s Hospital
No. 1, Ho-Chi Minh University, Ho Chi Minh City, Vietnamb; Blood Systems Research Institute, San Francisco, California, USAc; and Division of Microbiology, Department of
Pathology and Microbiology, Nihon University School of Medicine, Oyaguchi Kamicho, Itabashi-ku, Tokyo, Japand
cially children (2). Annually, rotavirus causes 611,000 deaths
worldwide (9). Rotavirus is composed of double-stranded RNA
including 11 segments: the structural proteins of VP1 to VP4,
VP6, and VP7 and the nonstructural proteins of NSP1 to NSP6.
Since VP7 and VP4 are related to the binding to specific receptors
in hosts and they contain neutralizing epitopes, a rotavirus classi-
VP4 (2, 5, 11). Recent reports suggest characterization of all rota-
virus segments in order to correctly identify the genetic diversity
and infectious source of the virus (7, 8).
children with acute gastroenteritis during January 2008 to July
2008 in Vietnam, a rare G10P rotavirus (strain 163/Vietnam)
was detected from a child. The complete coding regions of all 11
segments of this virus were sequenced by primer walking. Cycle
kit (Beckman Coulter Inc., Fullerton, CA), and then sequences
were analyzed on the CEQ 2000XL DNA analysis system (Beck-
2000XL DNA analysis system software (version 4.3.9; Beckman
constellation of G10P rotavirus was G10-P-I1-R1-C1-M1-
A1-N1-T1-E1-H1. Interestingly, this G10P rotavirus was
closely related to bovine rotavirus (strain VICG10.01) in the VP7
virus were highly identical to those of human G1P rotavirus
(strain Victoria/CK00047), sharing at least 99% identity at the
nucleotide level. Therefore, the 163/Vietnam strain had reassort-
ment between bovine and human rotaviruses.
genes derived from human rotavirus. The G10 rotavirus primarily
The detection of G10 rotavirus from human feces recently was re-
pathogen in causing acute gastroenteritis in humans, espe-
the 163/Vietnam strain was genetically closer to that of bovine G10
rotavirus (98% nucleotide identity) than to that of any human G10
rotaviruses (?89% nucleotide identity). The finding suggested that
the 163/Vietnam strain is a newly divergent human G10P rotavi-
layers (2). The VP7 is associated with interaction with neutralizing
In conclusion, we report the presence of a new human rotavi-
rus containing a sequence of bovine rotavirus. Continuous sur-
veillance is needed to understand the prevalence of this new virus
in causing acute gastroenteritis in Vietnam.
Nucleotide sequence accession numbers. The GenBank ac-
cession numbers for the complete coding regions of all 11 seg-
ments of the new human G10P rotavirus strain 163/Vietnam
are AB714258 to AB714268.
and Sciences and the Ministry of Health, Labor and Welfare, Japan.
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Received 22 June 2012 Accepted 26 June 2012
Address correspondence to Hiroshi Ushijima, firstname.lastname@example.org.
Y.M. and E.N. contributed equally to this study.
Copyright © 2012, American Society for Microbiology. All Rights Reserved.
jvi.asm.orgJournal of Virology p. 10236–10237 September 2012 Volume 86 Number 18
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