Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74)

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
The Scientific World Journal (Impact Factor: 1.73). 08/2012; 2012:578020. DOI: 10.1100/2012/578020
Source: PubMed


Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5 mm microshoots of
Oryza sativa
L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1 mg/L BAP, and 0.1 mg/L
-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1 mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds.

Download full-text


Available from: Noraini Mahmad, Dec 21, 2013
  • Source
    • "Illumination was at 1000 lux and relative humidity was 90 – 100%. The standard method for preparation of capsule matrix was followed [5]. "
    Dataset: 394-L00005

  • Source
    • "Illumination was at 1000 lux and relative humidity was 90 – 100%. The standard method for preparation of capsule matrix was followed [5]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Oxalis triangularis is an attractive ornamental plant propagated by using bulbs and has no natural viable seeds. It is an ideal decorative plant for growing in pots and other containers. The plant is not widely found in Malaysia and mostly obtained from Thailand. Hence, it is necessary to propagate this plant through tissue culture system for large scale production. In the present study, production of synthetic seeds was attempted from this species and the synthetic seeds managed to survive after 7 and 30 days after storage at 4°C. The rate of synthetic seeds conversion to complete plants after 7 days was 96.67% with 4.57 mean shoot numbers, while after 30 days of storage, the conversion rate slightly decreased to 90% with only 3.97 shoots formation per bead. The present work reports and discusses the potential of tissue culture technique as an alternative method for mass propagation and conservation of this interesting and attractive ornamental plant for future uses and exploitation.
    01/2013; 4(5):461-464. DOI:10.7763/IJESD.2013.V4.394