Article

Gene Sequencing for Routine Verification of Pyrazinamide Resistance in Mycobacterium tuberculosis: a Role for pncA but Not rpsA.

Public Health Ontario, Public Health Laboratories, Toronto, Ontario, Canada.
Journal of clinical microbiology (Impact Factor: 4.16). 08/2012; 50(11):3726-8. DOI: 10.1128/JCM.00620-12
Source: PubMed

ABSTRACT Pyrazinamide (PZA) is an important component of first-line therapy for the treatment of tuberculosis. Here, we evaluate targeted gene sequencing as a supplement to phenotypic PZA susceptibility testing of Mycobacterium tuberculosis. Routine sequencing of pncA, but not rpsA, is effective for verification of PZA susceptibility results.

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