Spectrophotometric and spectrofluorometric methods for the determination of non-steroidal anti-inflammatory drugs: A review

Arabian Journal of Chemistry (Impact Factor: 2.27). 01/2011;

ABSTRACT Non-steroidal anti-inflammatory drugs (NSAIDs) are the group most often used in human and veterinary medicine, since they are available without prescription for treatment of fever and minor pain. The clinical and pharmaceutical analysis of these drugs requires effective analytical procedures for quality control and pharmacodynamic and pharmacokinetic studies. An extensive survey of the literature published in various analytical and pharmaceutical chemistry related jour-nals has been conducted and the instrumental analytical methods which were developed and used for determination of some non-steroidal anti-inflammatory, coxibs, arylalkanoic acids, 2-arylpropi-onic acids (profens) and N-arylanthranilic acids (fenamic acids) in bulk drugs, formulations and biological fluids have been reviewed. This review covers the time period from 1985 to 2010 during which 145 spectrophotometric methods including UV and derivative; visible which is based on for-mation of metal complexation, redox reactions, ion pair formation, charge-transfer complexation and miscellaneous; flow injection spectrophotometry as well as spectrofluorometric methods were

1 Bookmark
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present paper describes an effective and low-cost spectrophotometric method for the determination of mefenamic acid in its pure form and pharmaceutical preparations. The method is based on the charge-transfer complexation between mefenamic acid as an n-electron donor and chloranil as a π-acceptor to form a violet chromogen measured at 540 nm. Under the optimum conditions, a linear relationship with a good correlation coefficient (0.9996) was found between the absorbance and concentration of the studied drug in the range of 10–60 μg/mL. The optimal reaction conditions such as reagent concentration, heating time, and stability of the reaction product were determined. The limit of detection (LOD) was 2.16 μg/mL and the limit of quantifycation (LOQ) was 7.15 μg/mL. The method was successfully applied to the determination of mefenamic acid in pharmaceutical preparations without any interference from common excipients.
    Journal of Analytical Chemistry 01/2008; 63(3):244-247. · 0.62 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Within the process of the International Conference on Harmonization (ICH), 2 guidelines were released containing a standardized terminology, a verified model of requirements for the validation of analytical procedures, and some guidance in the practical aspects of conducting validation studies in pharmaceutical analysis. For planar chromatographic procedures, which may be used at different levels either in qualitative identity testing, assays, semiquantitative limit tests, or quantitative determination of impurities, this paper tries to transfer these formal requirements into practical approaches for validation. Basic acceptance criteria for evaluation of validation experiments based on practical experience are proposed. In addition, selected parameters for robustness testing of given procedures and quality assurance of quantitative planar chromatographic testing by control charts is described.
    Journal of AOAC International 01/2001; 84(4):1265-76. · 1.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Diclofenac sodium (DS) is a drug with analgesic, antipyretic, and anti-inflammatory properties. It is pres-ent in numerous pharmaceutical preparations. In injectable forms, it is usually accompanied by benzyl alcohol (BA) as an excipient, which is used as a blocking anesthetic (4%) and an antiseptic (4-10%). In this work a spectrophotometric methodology was applied in order to determine benzyl alcohol and diclofenac in inject-able formulations by applying a multivariate calibration method. By a multivariate calibration method such as partial least squares (PLS), it is possible to obtain a model adjusted to the concentration values of the mixtures used in the calibration range. In this study, the concentration model is based on absorption spectra in the 230-320 nm range for 25 different mixtures of benzyl alcohol and diclofenac. Calibration matrix contains 10-95 and 1-50 mg mL -1 for benzyl alcohol and diclofenac, respectively. The root mean square errors of pre-diction (RMSEP) for benzyl alcohol and diclofenac were 3.0776 and 1.7557, respectively. The proposed method was validated by using a set of synthetic sample mixtures and subsequently applied to simultaneous determination of benzyl alcohol and diclofenac in two different pharmaceutical formulations.
    Journal- Chinese Chemical Society Taipei 01/2005; · 0.88 Impact Factor


Available from
Jun 5, 2014