Article

Detection of cross infections by Leishmania spp. and Trypanosoma spp. in dogs using indirect immunoenzyme assay, indirect fluorescent antibody test and polymerase chain reaction.

Faculdade de Odontologia, UNESP-Universidade Estadual Paulista, Rua Clóvis Pestana, 793, Araçatuba, São Paulo, CEP 16050-680, Brazil.
Parasitology Research (Impact Factor: 2.85). 07/2012; 111(4):1607-13. DOI: 10.1007/s00436-012-2999-2
Source: PubMed

ABSTRACT The aim of this study was to detect cross infections by Leishmania spp. and Trypanosoma spp. using enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and polymerase chain reaction (PCR). Thus, 408 blood samples were collected from dogs domiciled in Araçatuba Municipality, São Paulo State, Brazil; the dogs were of both sexes, of several breeds and aged 6 months. For Leishmania spp., 14.95 % (61 out of 408) of dogs were reactive using IFAT. Positivity was 20.10 % (82 out of 408) using ELISA and 29.66 % (121 out of 408) using PCR, with significant differences for the sex and age of these animals (p < 0.05). For Trypanosoma spp., antibody occurrence using ELISA was 10.54 % (43 out of 408), while PCR indicated 2.45 % (10 out of 408) positive dogs. Using IFAT, 10.29 % (42 out of 408) of animals were considered positive and only sex showed a significant difference (p < 0.05). In this study, 10.54 % (43 out of 408) of animals were seropositive according to ELISA for Trypanosoma spp., of which 79.07 % (34 out of 43) showed positive results in the molecular diagnosis for Leishmania spp., while of the 10.29 % (42 out of 408) positive dogs according to IFAT, 95.24 % (40 out of 42) had confirmed infection by this parasite. The obtained results demonstrate evidence of cross infections by both protozoa in the animals analysed in this study.

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