Article

Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/β-catenin signaling-reporter transgenic zebrafish.

Division of Cell Regulation Systems, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Developmental Biology (impact factor: 4.07). 07/2012; 370(1):71-85. DOI:10.1016/j.ydbio.2012.07.016 pp.71-85
Source: PubMed

ABSTRACT Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf7l1, and Tcf7l2) mediate gene expression regulated by Wnt/β-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/β-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/β-catenin signaling, which were expressed in known Wnt/β-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/β-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/β-catenin signaling-dependent processes.

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Keywords

anterior lateral line
 
embryogenesis
 
Evolutionarily conserved Tcf/Lef transcription factors
 
gene expression
 
gill filaments
 
multiple roles
 
new Wnt/β-catenin signaling reporter
 
organ development
 
organogenesis
 
reporter lines
 
secondary posterior lateral lines
 
spatiotemporal dynamics
 
stable transgenic zebrafish lines
 
Tcf/Lef-mediated Wnt/β-catenin signaling
 
Tcf/Lef-mediated Wnt/β-catenin signaling-dependent processes
 
tissue regeneration
 
transcriptional activities
 
transgenic lines
 
Wnt/β-catenin signaling
 
Wnt/β-catenin signaling-active sites