Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Department of Mechanical and Materials Engineering, Florida International University, United States.
Biosensors & Bioelectronics (Impact Factor: 6.45). 07/2012; 39(1):118-23. DOI: 10.1016/j.bios.2012.06.055
Source: PubMed

ABSTRACT The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

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May 19, 2014