Article

Photoluminescent and electrochemiluminescent dual-signaling probe for bio-thiols based on a ruthenium(II) complex

State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, PR China.
Analytica chimica acta (Impact Factor: 4.52). 08/2012; 740:80-7. DOI: 10.1016/j.aca.2012.06.028
Source: PubMed

ABSTRACT Photoluminescence (PL) and electrochemiluminescence (ECL) detection techniques are highly sensitive and widely used methods for clinical diagnostics and analytical biotechnology. In this work, a unique ruthenium(II) complex, [Ru(bpy)(2)(DNBSO-bpy)](PF(6))(2) (bpy: 2,2'-bipyridine; DNBSO-bpy: 2,4-dinitrobenzenesulfonate of 4-(4-hydroxyphenyl)-2,2'-bipyridine), has been designed and synthesized as a highly sensitive and selective PL and ECL dual-signaling probe for the recognition and detection of bio-thiols in aqueous media. As a thiol-responsive probe, the complex can specifically and rapidly react with bio-thiols in aqueous solutions to yield a bipyridine-Ru(II) complex derivative, [Ru(bpy)(2)(HP-bpy)](2+) (HP-bpy: 4-(4-hydroxyphenyl)-2,2'-bipyridine), accompanied by the remarkable PL and ECL enhancements. The complex was used as a probe for the PL and ECL detections of cysteine (Cys) and glutathione (GSH) in aqueous solutions. The dose-dependent PL and ECL enhancements showed good linear relationships against the Cys/GSH concentrations with the detection limits at nano-molar concentration level. Moreover, the complex-loaded HeLa cells were prepared for PL imaging of the endogenous intracellular thiols. The results demonstrated the practical utility of the complex as a cell-membrane permeable probe for PL imaging detection of bio-thiols in living cells.

0 Followers
 · 
158 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: A dinuclear ruthenium(II) complex linked via a reducible azo group [Ru(bpy)(2)(azobpy)Ru(bpy)(2)]Cl(4) (Ru(2)azo, bpy=2,2'-bipyridine, azobpy=4,4″-azobis (2,2'-bipyridine)) was adopted as a probe for thiols. Results showed that Ru(2)azo could selectively and effectively react with biological thiols (such as cysteine, homocysteine and glutathione) with a 10(-7)M detection limit. After it reacted with thiols, the original gray color of Ru(2)azo solution immediately turned yellow and the luminescence significantly enhanced, showing "naked-eye" colorimetric and "off-on" luminescent dual-signaling response for thiols. Mechanism studies demonstrated that Ru(2)azo reacted with thiols undergoing a two-electron transfer process, forming the azo(2-) anion product.
    Journal of inorganic biochemistry 01/2013; 121C:108-113. DOI:10.1016/j.jinorgbio.2012.12.019 · 3.27 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Based on an azo-thiol redox reaction, a non-emissive dinuclear iridium(iii) complex showed off-on phosphorescent response toward thiols and was developed to image thiol levels in living cells.
    Chemical Communications 02/2013; 49(20). DOI:10.1039/c3cc38687b · 6.72 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Microbicidal activity of hypochlorous acid (HOCl) plays pivotal roles in many biological processes, and suitable methods for the in vivo detection of HOCl are highly desirable. Herein we report the development of two novel ruthenium(II) complex-based phosphorescent chemosensors for HOCl, [Ru(bpy)2(DNCA-bpy)](PF6)2 (bpy: 2,2'-bipyridine; DNCA-bpy: 4-N-(2,4-dinitrophenyl)carboxamide-4'-methyl-2,2'-bipyridine) and [Ru(bpy)2(DNCH-bpy)](PF6)2 (DNCH-bpy: 4-N'-(2,4-dinitrophenyl)carbohydrazide-4'-methyl-2,2'-bipyridine). The two sensors are almost non-luminescent due to the intramolecular photoinduced electron transfer (PET) process from the Ru(II)-bpy moiety to dinitrophenyl group, but specific reaction of the sensors with HOCl in aqueous media can afford a highly luminescent derivative, [Ru(bpy)2(COOH-bpy)](PF6)2 (COOH-bpy: 4-carboxylic acid-4'-methyl-2,2'-bipyridine), accompanied by 190-fold and 1100-fold turn-on luminescence signal enhancement for [Ru(bpy)2(DNCA-bpy)](PF6)2 and [Ru(bpy)2(DNCH-bpy)](PF6)2, respectively. By taking advantage of high specificity and sensitivity of the chemosensor and excellent photophysical property of Ru(II) complex, [Ru(bpy)2(DNCH-bpy)](PF6)2 was applied for visualizing the endogenous HOCl generation in living macrophage cells during the processes of stimulation and phagocytosis via the red-emitting luminescence.
    Biosensors & Bioelectronics 06/2013; 50C:1-7. DOI:10.1016/j.bios.2013.06.005 · 6.45 Impact Factor
Show more