Thermodynamic behaviour in capillary electrochromatography
ABSTRACT The thermodynamic behaviour of solutes was examined on four reversed-phase (RP) stationary phases under pressure-driven (viz. high performance liquid chromatography, HPLC) and electro-driven (viz. capillary electrochromatography, CEC) conditions. Thermodynamic constants such as standard enthalpy, entropy, and Gibbs free energy were compared for both separation modes. Differences and/or similarities observed were used as a tool in gaining a better understanding of the separation mechanism in CEC compared to HPLC. In addition, effects of temperature on the magnitude of electroosmotic flow (EOF) are explained in detail.