Thermodynamic behaviour in capillary electrochromatography

Eindhoven University of Technology, Department of Chemical Engineering and Chemistry, Laboratory of Instrumental Analysis, P.O. Box 513, 5600 MB Eindhoven, The Netherlands
Journal of Separation Science (Impact Factor: 2.59). 06/2002; 25(9):569 - 576. DOI: 10.1002/1615-9314(20020601)25:9<569::AID-JSSC569>3.0.CO;2-3

ABSTRACT The thermodynamic behaviour of solutes was examined on four reversed-phase (RP) stationary phases under pressure-driven (viz. high performance liquid chromatography, HPLC) and electro-driven (viz. capillary electrochromatography, CEC) conditions. Thermodynamic constants such as standard enthalpy, entropy, and Gibbs free energy were compared for both separation modes. Differences and/or similarities observed were used as a tool in gaining a better understanding of the separation mechanism in CEC compared to HPLC. In addition, effects of temperature on the magnitude of electroosmotic flow (EOF) are explained in detail.

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    ABSTRACT: Capillary electrochromatography (CEC) is a miniaturized separation technique that combines aspects of both interactive chromatography and capillary electrophoresis. In this chapter, the theory of CEC and the factors affecting separation such as the stationary phase and mobile phase parameters have been discussed. The chapter focuses on the types and preparation of columns for CEC and describes the progress made in the development of open-tubular, particle-packed, and monolithic columns. The detection techniques in CEC such as the traditional UV detection and improvements made in coupling with more sensitive detectors such as mass spectrometry are also described. The chapter provides a summary of some applications of CEC in the analysis of pharmaceuticals and biotechnology products.
    Separation Science and Technology 9:439-476. DOI:10.1016/S0149-6395(07)00017-7 · 1.16 Impact Factor