Article

An Oxygen‐Insensitive Reagentless Glucose Biosensor Based on Osmium‐Complex Modified Polypyrrole

Electroanalysis (Impact Factor: 2.82). 12/2000; 12(17):1383 - 1389. DOI: 10.1002/1521-4109(200011)12:17<1383::AID-ELAN1383>3.0.CO;2-0

ABSTRACT An optimized material for the development of reagentless oxygen-independent biosensors based on conducting polymers is described. Considering the prerequisites for a fast electron transfer between a redox enzyme and the electrode surface via an electron-hopping mechanism, an Os-complex-modified pyrrole derivative with a long, flexible spacer chain has been synthesized. Copolymerization of the new mediator-modified pyrrole monomer with pyrrole was optimized aiming on a higher mediator loading in the film. The feasibility of this material for the development of reagentless oxygen-independent biosensors is demonstrated by entrapment of a PQQ-dependent glucose dehydrogenase isolated from Erwinia sp. 34-1 within this electrochemically grown redox-polymer network.

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May 26, 2014