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An Oxygen‐Insensitive Reagentless Glucose Biosensor Based on Osmium‐Complex Modified Polypyrrole

Electroanalysis (Impact Factor: 2.82). 12/2000; 12(17):1383 - 1389. DOI: 10.1002/1521-4109(200011)12:17<1383::AID-ELAN1383>3.0.CO;2-0

ABSTRACT An optimized material for the development of reagentless oxygen-independent biosensors based on conducting polymers is described. Considering the prerequisites for a fast electron transfer between a redox enzyme and the electrode surface via an electron-hopping mechanism, an Os-complex-modified pyrrole derivative with a long, flexible spacer chain has been synthesized. Copolymerization of the new mediator-modified pyrrole monomer with pyrrole was optimized aiming on a higher mediator loading in the film. The feasibility of this material for the development of reagentless oxygen-independent biosensors is demonstrated by entrapment of a PQQ-dependent glucose dehydrogenase isolated from Erwinia sp. 34-1 within this electrochemically grown redox-polymer network.

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    ABSTRACT: The design of polymers carrying suitable ligands for coordinating Os complexes in ligand exchange reactions against labile chloro ligands is a strategy for the synthesis of redox polymers with bound Os centers which exhibit a wide variation in their redox potential. This strategy is applied to polymers with an additional variation of the properties of the polymer backbone with respect to pH-dependent solubility, monomer composition, hydrophilicity etc. A library of Os-complex-modified electrodeposition polymers was synthesized and initially tested with respect to their electron-transfer ability in combination with enzymes such as glucose oxidase, cellobiose dehydrogenase, and PQQ-dependent glucose dehydrogenase entrapped during the pH-induced deposition process. The different polymer-bound Os complexes in a library containing 50 different redox polymers allowed the statistical evaluation of the impact of an individual ligand to the overall redox potential of an Os complex. Using a simple linear regression algorithm prediction of the redox potential of Os complexes becomes feasible. Thus, a redox polymer can now be designed to optimally interact in electron-transfer reactions with a selected enzyme. Figure A library of redox electrodeposition polymers was synthesized and the formal potentials of the polymer-bound Os-complexes were adjusted through variations of the coordination shell. Optimal adaptation to the redox potentials of enzymes could be attained
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    ABSTRACT: Publications in fields of Biosensors, conducting polymers etc.

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