Article

Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

Department of Science and Technology Centre on Biomolecular Electronics, National Physical Laboratory, (Council of Scientific and Industrial Research), Dr. K. S. Krishnan Marg, New Delhi-110012, India phone: +91-11-45609152, fax: 45609310
Electroanalysis (impact factor: 2.87). 08/2010; 22(22):2683 - 2693. DOI:10.1002/elan.201000269 pp.2683 - 2693

ABSTRACT Nanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium-tin-oxide (ITO) coated glass plate using electrophoretic technique. Co-immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP-GDH/PANI-SWCNT-TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL−1 with low Michaelis–Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28×10−4 mA mg−1 dL and storage stability of about 13 weeks.

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Keywords

electrophoretic technique
 
GDH
 
ITO
 
linear sweep voltammetry revealing
 
LIP-GDH/PANI-SWCNT-TB/ITO bioelectrode
 
low Michaelis–Menten constant
 
N-ethyl-N′-(3-dimethylaminopropyl)
 
N-hydroxysuccinimide chemistry
 
Nanocomposite film
 
Response studies
 
response time
 
single walled carbon nanotubes
 
storage stability