Article

Surface Plasmon Enhanced TIRF Imaging

Langevin Institute, ESPCI, Paris, France
Imaging & Microscopy 10/2009; 11(4):55 - 56. DOI:10.1002/imic.200990091 pp.55 - 56

ABSTRACT Total Internal Reflection Fluorescence Microscopy (TIRFM) is a powerful technique for imaging dynamic membrane events in living cells. However the information gained from TIRFM may be limited by the intensity of the florescence signal and by background noise emanating from the inner part of the cell. Here we describe how Surface Plasmon Mediated Fluorescence Microscopy (SPMFM) can enhance TIRFM by increasing the fluorescence signal 5-6 fold and by reducing background noise dramatically. The imaging configuration is that for TIRFM except that the coverglass is coated on one side with a nanometre film of silver. For SPMFM (as in TIRFM), a high numerical aperture (N.A.) objective is essential for achieving required illumination angles.

0 0
 · 
0 Bookmarks
 · 
18 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

background noise
 
background noise emanating
 
florescence signal
 
fluorescence signal 5-6
 
illumination angles
 
imaging dynamic membrane events
 
inner part
 
SPMFM
 
Surface Plasmon Mediated Fluorescence Microscopy
 
Total Internal Reflection Fluorescence Microscopy
 

Karla Balaa