Genistein and Daidzein Concentrations and Contents in Seedling Roots of Three Soybean Cultivars Grown under Three Root Zone Temperatures
ABSTRACT Daidzein and genistein are plant-to-bacterium signal compounds involved in soybean nodule formation. They can induce nod gens expression in Bradyrhizobium japonicum. The objective of this study was to determine whether the production of signal molecules was affected by low root zone temperatures (RZTs) in a manner that varied among soybean cultivars. Daidzein and genistein concentrations of soybean seedling roots were measured at three RZTs by high performance liquid chromatography (HPLC). The results indicated that daidzein content and concentration per plant were higher at 15 and 17.5°C than those at 25°C. AC Bravor had higher daidzein contents and concentrations than did Maple Glen and KG20. At 17.5°C. KG20 had higher genistein content and concentration levels than Maple Glen, and no difference existed for the two cultivars at 15 and 25 C. Daidzein contents and concentrations of Maple Glen and AC Bravor increased with harvest time. However, for cultivar KG20, the content and concentration decreased at 19 days after inoculation. Genistein contents and concentrations of the three cultivars increased under each RZT up to the last harvest. There was an interaction between soybean cultivar and RZT for root genistein and daidzein contents and concentrations. The content and concentration of daidzein in soybean seedling roots were much higher (more than five times) than those of genistein.
- SourceAvailable from: Takuji OhyamaAdvances in Biology and Ecology of Nitrogen Fixation, First edited by Takuji Ohyama, 01/2014: chapter Chapter 7: Impact of Harch Environmental Conditions on Nodule Formation and Dinitrogen Fixation of Legumes.: pages 131-193; InTech., ISBN: 978-953-51-1216-7
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ABSTRACT: The plant molecular signals that induce legume-Rhizobium symbiosis are potentially an important source of plant growth promotion in agricultural systems. A study was conducted to evaluate the effects of low root zone temperature (RZT) on nitrogen fixation, antioxidant response, and growth of the lentil plant (Lens culinaris L.), which harbor isoflavonoids that may exert positive impacts on human health, inoculated with R. leguminosarum bv. viciae pre-cultured with additional plant-torhizobia signal compounds (inducers), naringenin (NA), methyl-jasmonate (MJ), and NA+MJ treatment, under greenhouse conditions. Two RZT levels, 20 and 14oC, were maintained through daily irrigations until the plants were harvested 25–30 days after transplantation. The addition of inducer compounds increased the dry weight and leaf greenness of plants under both temperature regimes. Photosynthesis levels were increased significantly (p<0.05) by approximately 12.3% when compared to the group without inducer treatment (control). Nitrogen fixation per plant increased with increasing numbers of nodules. The fixed nitrogen per plant in the NA and NA+MJ treatment groups increased by 18.2 and 20.4% as compared to the controls. No interaction between inducer and temperature factors was detected. Increasing low RZT levels significantly (p<0.05) reduced antioxidant activity, proline content, ascorbate peroxidase, and glutathione reductase contents in the plants treated with induced rhizobia as compared to the controls. Phenol content was recorded as higher at a temperature of 14 than at 20oC, and was significantly (p<0.05) increased in the inducer treatments. These results, collectively, suggest that the pre-incubation of bacterial cells with plant-to-bacteria signal compounds could enhance lentil growth, photosynthetic rates, and nitrogen fixation, and could also alleviate low RZT stress.Journal of the Korean Society for Applied Biological Chemistry 01/2009; 52(6):688-693. · 0.43 Impact Factor
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ABSTRACT: Soil stresses such as salinity and acidity may adversely affect nitrogen (N)2-fixation. The hypothesis of this study is that soil salinity and acidity inhibit soybean [Glycine max (L.) Merr.] nodulation and N2-fixation due to, at least in part, disruption of the signal exchange process. The objectives were: 1) to determine the effects of stressful soil salinity and acidity on the signal exchange processes between soybean and Bradyrhizobium japonicum, and 2) to determine whether or not the addition of signal molecule genistein to B. japonicum can overcome at least part of the inhibition of nodulation, caused by stressful soil salinity and acidity. Salt (sodium chloride) and sulfur (S) were applied. Genistein (0, 5, and 20μ M) was tested. Genistein addition could partially overcome the salt and acidity stresses by increasing soybean yields up to 21% and 23%, respectively. These novel findings may be very useful for planting soybean under salinity and acidity stresses.Journal of Plant Nutrition 01/2007; 30(12):1967-1992. · 0.53 Impact Factor