First notification on the presence of brucellosis in water buffalo (Bubalus bubalis) in Mexico by serological tests

African journal of microbiology research (Impact Factor: 0.54). 05/2012; 6(13):3242-3247. DOI: 10.5897/AJMR11.1630


A cross-sectional study was conducted to determine the seroprevalence of brucellosis in water buffaloes (Bubalus bubalis) of three farms located in the south of Veracruz, México. Card test and rivanol were used in serial for detection of antibodies against Brucella spp. From a total of 565 buffaloes, 99 were tested and the overall seroprevalence of brucellosis was 13% by card test and 7% by rivanol. By farm, seroprevalence was 2.94% for farm 1. 4% for farm 2, and 12.5% for farm 3. Brucellosis seroprevalence showed an increasing trend with age, with adult cattle (>6 years) recording the highest seroprevalence (11.1%), but differences were not statistically significant (p>0.05). In two farms, buffaloes shared grazing land and water sources with bovine cattle, so 75 head were tested for brucellosis resulting negative. Because clinical signs suggestive of brucellosis were not observed, isolation was not attempted. This is the first known report on the presence of brucellosis in water buffalo in Mexico; thus, public awareness and further epidemiological studies of the disease in wildlife, livestock, and humans in the study area are of great importance.

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Available from: José Alfredo Villagómez-Cortés,
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    • "Toxoplasmosis in humans may be a severe disease affecting the lymph nodes, eyes, and central nervous system and can be life-threatening mostly in immunocompromised patients. Water buffaloes are important to the economy of many countries and in Mexico they represent an emerging alternative for diversified livestock production [2]. In Asia, water buffaloes are the major source of milk and milk products, and their meat is consumed by humans in several countries. "
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    ABSTRACT: Background Infection with Toxoplasma gondii in water buffaloes (Bubalus bubalis) is of epidemiological importance because of the risk for transmission to humans. We sought to determine the seroprevalence of T. gondii infection in 339 water buffaloes in Veracruz State, Mexico using the modified agglutination test (MAT, cut off 1:25). Seroprevalence association with general characteristics of buffaloes and their environment was also investigated.ResultsAntibodies to T. gondii were found in 165 (48.7%) of the 339 buffaloes with MAT titers of 1:25 in 104, 1:50 in 52, and 1:100 in 9. Bivariate analysis showed that seroprevalence of T. gondii infection was similar in buffaloes regardless of their general characteristics i.e., age, sex, and breed. In contrast, the seroprevalence in buffaloes varied significantly with environmental characteristics including altitude, mean annual temperature, and mean annual rainfall of the municipalities studied. Multivariate analysis showed that T. gondii seropositivity in buffaloes was associated with a mean annual rainfall between 1266¿1650 mm (OR =1.84; 95% CI: 1.15-2.94; P¿=¿0.01).Conclusions Results indicate that environmental characteristics may influence the seroprevalence of T. gondii infection in buffaloes. This is the first report on the seroprevalence of T. gondii infection in buffaloes in Mexico. Further research is needed to assess the risk for infection in humans associated with the ingestion of raw or undercooked meat from buffaloes infected with T. gondii.
    BMC Veterinary Research 09/2014; 10(1):232. DOI:10.1186/s12917-014-0232-5 · 1.78 Impact Factor