Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India*
ABSTRACT SummaryA procedure for the purification of a tospovirus which causes bud necrosis disease (BND) of peanut in India is described. The virus contained three polypeptides of 78 kDa, 54 kDa and 31 kDa. In two ELISA procedures the virus failed to react with antisera to tomato spotted wilt virus (TSWV) obtained from different sources and with an antiserum to impatiens necrotic spot virus (INSV). Additionally, in reciprocal tests TSWV and INSV antigens failed to react with antiserum to the virus infecting peanut in India.In electro-blot immunoassay 54 kDa and 31 kDa polypeptides of the virus reacted with the homologous antiserum. None of the heterologous antisera reacted with any of the three viral polypeptides. On the basis of serological differences the virus that causes BND in India is distinct and therefore has been named bud necrosis virus (BNV). This serotype appears to be restricted to Asia.
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ABSTRACT: Sterility mosaic (SMD) is the most damaging disease of pigeonpea (Cajanus cajan (L.) Millsp.) in the Indian subcontinent. After seven decades of research, vital breakthroughs made on the identification, detection, and transmission of the causal agent of this major disease are enabling the development of broad-based durable resistant pigeonpea cultivars. These breakthroughs will contribute greatly to sustainable pigeonpea production and enhance the income and livelihood of poor farmers in the semiarid tropics of the Indian subcontinent.Plant Disease 01/2004; 88(5):436-445. · 2.74 Impact Factor
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ABSTRACT: Genetic engineering of peanut (Arachis hypogaea L.) using the gene encoding for the nucleocapsid protein (N gene) of peanut bud necrosis virus (PBNV; genus Tospovirus, family Bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (PBND), a disease for which no durable resistance is available in the existing germplasm. Over 200 transgenic lines of peanut var. JL 24 were developed for which integration and expression of the transgenes was confirmed by PCR, Southern hybridization, RT-PCR and western blot analysis. The T(1) and T(2) generation transgenic plants were assayed through virus challenge in the greenhouse by using mechanical sap inoculation at 1:100 and 1:50 dilutions of PBNV, and they showed varying levels of disease incidence and intensity. Greenhouse and field evaluation with T(2) generation plants indicated somewhat superior performance of the three transgenic events that showed considerable reduction in disease incidence. However, only one of these events showed over 75 % reduction in disease incidence when compared to the untransformed control, indicating partial and non-durable resistance to PBND using the viral N-gene.Archives of Virology 09/2012; · 2.28 Impact Factor
Article: Plant Viruses Transmitted by Thrips[Show abstract] [Hide abstract]
ABSTRACT: All thrips (order Thysanoptera) that are known to be vectors of plant viruses are identified and described. Thrips transmit plant viruses in the Tospovirus, Ilarvirus, Carmovirus, Sobemovirus and Machlomovirus genera. Tospoviruses are the cause of a number of significant emerging diseases, such as capsicum chlorosis and scape blight of onion. They infect thrips as well as plant hosts and the relationship between pathogen and vector is intimate. Once infected at the larval stage, adult thrips usually transmit tospovirsuses for life. Transmission to plant hosts occurs when thrips feed. Information on the distribution and hosts of all recognised thrips vectors is provided. Fourteen tospovirus species are described with information provided on other tospoviruses that have not yet been designated as species. The history of the research that has led to present knowledge is reviewed in chronological order for each tospovirus. The possible origin of tospoviruses is discussed. Information is presented on viruses, which are thrips-transmitted by mechanical processes, in other genera. Pathways of spread of thrips vectors in relation to the threat of tospoviruses to European agriculture are discussed.European Journal of Plant Pathology 01/2005; 113(2):119-157. · 1.71 Impact Factor