Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India*

University of Idaho, Москва, Idaho, United States
Annals of Applied Biology (Impact Factor: 1.96). 02/2008; 120(2):279 - 286. DOI: 10.1111/j.1744-7348.1992.tb03425.x

ABSTRACT SummaryA procedure for the purification of a tospovirus which causes bud necrosis disease (BND) of peanut in India is described. The virus contained three polypeptides of 78 kDa, 54 kDa and 31 kDa. In two ELISA procedures the virus failed to react with antisera to tomato spotted wilt virus (TSWV) obtained from different sources and with an antiserum to impatiens necrotic spot virus (INSV). Additionally, in reciprocal tests TSWV and INSV antigens failed to react with antiserum to the virus infecting peanut in India.In electro-blot immunoassay 54 kDa and 31 kDa polypeptides of the virus reacted with the homologous antiserum. None of the heterologous antisera reacted with any of the three viral polypeptides. On the basis of serological differences the virus that causes BND in India is distinct and therefore has been named bud necrosis virus (BNV). This serotype appears to be restricted to Asia.

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    ABSTRACT: Peanut bud necrosis virus (PBNV), genus Tospovirus (family Bunyaviridae), is an important virus infecting peanut and other crops in South India. PBNV isolates naturally infecting groundnut, brinjal, tomato, black gram, field bean, cowpea, cotton, jute, taro and Calotropis plants were collected from different regions of South India and characterized. Infection was confirmed by direct antigen-coating enzyme-linked immunosorbent assay (DAC-ELISA) using PBNV-specific antiserum. The coat protein gene was further amplified using PBNV coat protein-specific primers. The amplicon (830 bp) was cloned and sequenced; sequence analysis revealed that the N gene shared 93–100% and 95–100% sequence identity with PBNV at the nucleotide and amino acid levels, respectively.
    Journal of Phytopathology 12/2013; 162(7-8). DOI:10.1111/jph.12219 · 0.92 Impact Factor
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    ABSTRACT: Tospoviruses belong to the sole phytovirus genus, Tospovirus, in the family Bunyaviridae. Tospoviruses are known to be exclusively transmitted by thrips belonging to the family Thripidae and subfamily Thripinae. Of the known 1,710 species of Thripidae only 14 thrips species are currently reported to transmit tospoviruses. Thrips-transmitted tospoviruses cause severe yield losses to several economically important crops in the United States and worldwide. For instance, a single Tospovirus (Tomato spotted wilt virus) alone caused an estimated $1.4 billion in losses in the U.S. over 10 years. Global trade and associated movement of plant materials across borders have introduced tospoviruses and their vectors into newer areas. Advances in serological and molecular techniques have also led to identification of new tospoviruses. This scenario has also initiated new vector-pathogen interactions between introduced and native thrips species and tospoviruses. The goal of this manuscript is to provide a comprehensive and updated list of thrips species that serve as vectors of tospoviruses along with information pertaining to common names, key diagnostic characters, distribution, important crops economically affected, and thrips and Tospovirus-induced symptoms. The manuscript is prepared with special emphasis to the U.S., but information pertaining to other countries is also included.
    03/2011; 2(1):I1-I10. DOI:10.1603/IPM10020
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    ABSTRACT: Groundnut or peanut is an important annual oilseed and food legume crop grown in diverse environments throughout the tropical world. About 31 viruses representing 14 genera were reported to naturally infect groundnut in different parts of the world. Among these, Tomato spotted wilt virus (TSWV), Groundnut bud necrosis virus (GBNV), Tobacco streak virus (TSV), Groundnut rosette assistor virus (GRAV), Groundnut rosette virus (GRV), satellite RNA associated with GRV and/or GRAV, Peanut clump virus (PCV), Peanut stripe virus (PStV), a strain of Bean common mosaic virus (BCMV), Peanut mottle virus (PeMoV) and Cucumber mosaic virus (CMV) have either global / regional economic importance. Cowpea aphid-borne mosaic virus (CABMV), CMV, IPCV, PCV, PeMoV, PStV, PSV and Cowpea mild mottle virus (CPMMV-severe strain) are transmitted through groundnut seed and thus are important quarantine pests. A review on the distribution, incidence, symptoms, impact on crop growth and yield, virus properties, phylogenetic relationships, virus detection, epidemiology and management of TSWV, GBNV, TSV, rosette complex, peanut clump, PStV, PeMoV and CMV, is presented in this chapter.
    Edited by G.P. Rao, 01/2008; Studium Press., ISBN: 1-933699-31-0