Article

[Study on the effect of grape seed proanthocyanidins on increasing the roadiosensitivity for X-ray].

Stomaological hospital, Lanzhou university, Lanzhou 730000, China.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 02/2012; 35(2):264-9. pp.264-9
Source: PubMed

ABSTRACT To investigate the effect of Grape Seed Proanthocyanidins (GSPs) on enhancing the radiosensitivity of human hepatic carcinoma cell line HepG2, human cervical cancer cell line Hela and human leukemia cell line K562 for X-ray in vitro.
The killing effect of GSPs combined with X-ray on cells was evaluated by SRB and clone formation assay.
The GSPs had obvious cytotoxicity on three cell lines in a dose-dependent and time-dependent manners. However, inhibition rate of different cell line were quite different, the strongest one was human leukemia K562 cells and the others were weak. The sensitization ranges calculated by univariate analysis were 6.25-12.5 microg/mL for human leukemia K562 cells. Sensitization enhancement ratio was 1.94 using curve fitting method for K562 cells.
GSPs can obviously enhance the radiosensitivity of cancer cells in vitro. The mechanism of sensitization effect may be related to the effects of GSPs on oxygen balance and cell cycle.

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Keywords

cancer cells
 
cell cycle
 
cell lines
 
curve fitting method
 
different cell line
 
dose-dependent
 
Grape Seed Proanthocyanidins
 
human cervical cancer cell line Hela
 
human hepatic carcinoma cell line HepG2
 
human leukemia cell line K562
 
human leukemia K562 cells
 
inhibition rate
 
others
 
oxygen balance
 
radiosensitivity
 
sensitization effect
 
Sensitization enhancement ratio
 
sensitization ranges
 
time-dependent manners
 
univariate analysis
 

Xiao-Jing Pan