Flavonoids: Potent inhibitors of poliovirus RNA synthesis
ABSTRACT Some naturally occuring flavonoids, such as 3-methyl quercetin and Ro-090179, show potent anti-picornavirus activity. They inhibit poliovirus replication at concentrations 100-fold or 1000-fold lower than hydroxybenzyl-benzimidazole (HBB) and guanidine, respectively. Ro-090179 selectively blocks viral RNA synthesis in poliovirus-infected HeLa cells more strongly than 3-methyl quercetin and is therefore the most potent and selective inhibitor of poliovirus RNA synthesis described until now. In addition, Ro-090179 discriminates in its inhibition between plus- and minus-stranded RNA synthesis. Thus, analysis of the viral RNA made in poliovirus-infected cells when the compound is added late in the infection cycle, indicates that the synthesis of genomic RNA is potently blocked, whereas minus-stranded RNA synthesis is not inhibited. These findings make Ro-090179 a valuble compound for obtaining insight into the molecular mechanism of poliovirus RNA replication.
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ABSTRACT: Avicenniaceae family is a member of true mangrove plants which has one genus, 11 species and several subspecies. Avicennia marina is the most current species among these plants in Iranian mangrove forest. Regarding to the presence of many active biological constituents in this plant and their applications in traditional and alternative medicine, the in vitro antiviral activity of its leaf extract on herpes simplex virus type-1 (HSV-1) and vaccine strain of polio virus (Sabin) in Vero cell line were determined. The CC50 of the extract was 5750.96 for Vero cells. The antiviral effect of the extract on HSV-1 and vaccine strain of polio virus before and after the attachment of the virus particles to Vero cells were assessed. The IC50 values of the extract were 66 µg/ml and 137.24 µg/ml for before and after virus attachment stages of HSV-1 replication cycle respectively. The IC50 values of extract for vaccine strain of poliovirus were 145.7 and 314.3 µg/ml for before and after attachment stages of virus replication respectively. The SI values of the extract for the before and after virus attachment stages of viral replication cycle were 87.1 and 41.9 for HSV-1. The SI for the vaccine strain of poliovirus were calculated 39.5 and 18.3 for before and post attachment stages of this virus replication cycle ordinarily. The obtained SI values indicate that hot glycerin extract of A. marina leaves could be a good candidate for further studies in the area of antiviral compound developing.Journal of Medicinal Plants Research. 11/2009; 3:771-775.
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ABSTRACT: Fourteen complexes of di-n-butyltin(IV)(2+) cations with flavonoid glycosides (rutin, hesperidin, 2',4',3-trihydroxy-5',4-dimetoxychalkone 4-rutinoside) and flavonoid aglycones (quercetin, morin, hesperitin and sorte flavones) were prepared. The composition of the complexes was determined by standard analytical methods. The results showed that complexes containing diorganotin(lV)(2+) moiety and the ligand in 1:1, 2:1 or 3:1 ratio are formed. The FTIR spectra were consistent with the presence of Sn-O (phenol or carbohydrate) vibration in the compounds. The structure of the complexes was measured by Mossbauer spectroscopy. Comparison of the experimental quadrupole splitting with those calculated on the basis of partial quadrupole splitting concept revealed that the complexes are of four types: with the central tin atoms surrounded by donor atoms in a purely trigonal-bipyramidal, octahedral+trigonal-bipyramidal, trigonal-bipyramidal+tetrahedral and octahedral+tetrahedral arrangement. This procedure also distinguished between the different structural isomers of both trigonal-bipyramidal and octahedral complexes. Conclusions could therefore be drawn on the factors determining which of the isomers are formed in the systems. The Mossbauer parameters obtained for organotin(rv)-flavonoid complexes were compared with those measured for organotin(IV)-carbohydrate complexes.Journal of Radioanalytical and Nuclear Chemistry 01/1998; 227(1-2):89-98. · 1.41 Impact Factor
- Natural Product Reports 09/2010; 27(11):1571-93. · 10.72 Impact Factor