Article

A promising inert support for laccase production and decolouration of textile wastewater by the white-rot fungus Trametes pubescesns

CEIT, Unit of Environmental Engineering, San Sebastian, Spain.
Journal of hazardous materials (Impact Factor: 4.33). 07/2012; 233-234:158-62. DOI: 10.1016/j.jhazmat.2012.07.003
Source: PubMed

ABSTRACT Cubes of nylon sponge, cubes of polyurethane foam (PUF), cuttings of stainless steel sponges and the commercial carriers Kaldnes™ K1 were tested as inert supports for laccase production by the white-rot fungus Trametes pubescens under semi-solid-state fermentation conditions. The cultures operating with Kaldnes™ K1 led to the highest laccase activity (3667 U/l). In addition this support could be re-utilised, making the whole process more economical. Subsequently, the decolouration of simulated textile wastewater (STW) by T. pubescens grown on the different tested supports under semi-solid-state fermentation conditions was studied. Decolouration percentages around 66-80% were obtained in 96 h. It was found that STW decolouration was due to two mechanisms: laccase action (biodegradation) and adsorption onto fungal mycelium, save for the PUF cultures in which decolouration was mainly due to adsorption onto the support. Further, the decolouration of STW by Kaldnes™ K1 cultures in three successive batches of 96 h each was studied. Decolouration percentages of 51.3, 70.0 and 69.8%, were attained for each batch, respectively.

Download full-text

Full-text

Available from: Susana Rodriguez-Couto, Jun 27, 2015
1 Follower
 · 
265 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The decolouration of the metal-complex dyes Bemaplex Navy M-T (150 mg/L) and Bezaktiv Blue BA (150 mg/L) in nine successive batches by immobilised cultures of the white-rot fungus Trametes pubescens was studied. Two different types of immobilisation supports were used: the commercial carriers Kaldnes™ K1 (synthetic supports) and sunflower-seed shells (SS) (natural supports). Bemaplex showed more resistance to degradation by T. pubescens cultures than Bezaktiv, especially in the K1 cultures. Thus, SS cultures led to decolouration percentages higher than 59% for Bemaplex in all the batches save for the last two and higher than 50% for Bezaktiv in all the batches except for the 2nd and 9th ones. K1 cultures showed decolouration percentages for Bemaplex higher than 42% in batches 1, 3, 4, 5 and 7 and for Bezaktiv higher than 70% in all the batches save for the last one. Dye decolouration was mainly due to enzyme action (biodegradation).
    10/2014; 4. DOI:10.1016/j.btre.2014.10.006
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The production of neomycin by the actinomycete Streptomyces fradiae, under semi-solid-state fermentation conditions was the main subject of this study. Two supports (nylon sponge and orange peelings) were tested in order to determine the most suitable one for the production of neomycin by the above-mentioned microorganism. Nylon sponge led to the highest neomycin production, reaching a maximum value of 13,903μg/mL on the 10(th) day of cultivation. As a control, the same experiment was performed under submerged fermentation (SmF) conditions, without solid support. Here the production of neomycin by S. fradiae was about 55-fold lower (i.e. 250μg/mL) than that obtained for SSF.
    Journal of Biotechnology 04/2013; 165(3-4). DOI:10.1016/j.jbiotec.2013.03.015 · 2.88 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: White-rot fungal strains of Trametes versicolor and Phanerochaete chrysosporium were selected to study the decolourisation of the textile dye, Reactive Black 5, under alkaline-saline conditions. Free and immobilised T. versicolor cells showed 100 % decolourisation in the growth medium supplemented with 15 g l(-1) NaCl, pH 9.5 at 30 °C in liquid batch culture. Continuous culture experiments were performed in a fixed-bed reactor using free and immobilised T. versicolor cells and allowed 85-100 % dye decolourisation. The immobilisation conditions for the biomass and the additional supply of carbon sources improved the decolourisation performance during a long-term trial of 40 days. Lignin peroxidase, laccase and glyoxal oxidase activities were detected during the experiments. The laccase activity varied depending on carbon source utilized and glycerol-enhanced laccase activity compared to sucrose during extended growth.
    Current Microbiology 08/2013; 68(1). DOI:10.1007/s00284-013-0441-3 · 1.36 Impact Factor