Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem 23, 882-884
ABSTRACT We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.
Full-textDOI: · Available from: John A Colwell, Feb 19, 2015
- SourceAvailable from: Dahlia Badran
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- "HDL-C was measured using Roche/Hitachi HDL-C kit (Cat. number 03030024) and was estimated by phosphotungstic acid precipitation followed by enzymatic analysis in supernatant fraction . LDL-C was calculated by the Friedewald formula after considering its limitations if TG > 400 mg/dL: LDL-C = TC − (HDL-C + TG/5) . "
ABSTRACT: Background. Beta 2 -adrenergic receptor (ADRB2) gene polymorphisms, Arg16Gly and Gln27Glu, have been implicated in the pathogenesis of cardiovascular diseases. The aim of this study was to determine the association of these two polymorphisms with the risk of myocardial infarction (MI) in the Egyptian population. Methods. Blood samples were collected from 68 MI patients and 75 healthy controls. They were assessed for the presence of cardiovascular risk factors and genotyped for the Arg16Gly (rs1042713) and Gln27Glu (rs1042714) polymorphisms using allelic-discrimination polymerase chain reaction. Results. There is no significant difference in genotype and allele frequencies at codon 16 between MI patients and controls (íµí± = 0.919). However, at codon 27, MI risk was higher in Gln 27 homozygous participants than in Glu 27 carriers (íµí± = 0.045). The haplotype frequency distribution showed significant difference among cases and controls (íµí± = 0.002); homozygotes for Gly 16 /Gln 27 haplotype were more susceptible to MI than Gly 16 /Glu 27 carriers. Patients with Arg 16 /Gln 27 haplotype had higher serum total cholesterol levels (íµí± < 0.05) and lower frequency of diabetes in MI patients (íµí± < 0.01). However, both Glu 27 genotypes and haplotype showed lower frequency of hypertension (íµí± < 0.001). Conclusions. Our findings suggested that the ADRB2 gene polymorphisms may play an important role in susceptibility of MI among Egyptian population.12/2014; DOI:10.1155/2014/471635
- "The blood glucose level was determined by glucose oxidase-peroxidase method using a kit Monozyme India limited, Ahmadabad (Trinder, 1969). Total cholesterol, triglyceride and HDLcholesterol were estimated by CHOD-PAP (Stockbridge et al., 1989), triglycerides (Fossati and Prencipe, 1982) and HDL-Cholesterol (Lopes-virella et al., 1977) was estimated by spectrophotometric assays employing commercially available kits. LDL and VLDL were calculated from Freidewald's formula. "
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- "µl of reagent present in kit mixed samples and incubated it for 10 minutes at 25°C and absorbance was read at 500 nm of the standard and samples against reagent blanks within 60 minutes, for examination of serum cholesterol level, Samples were prepared by taking 10µl of serum in small glass bottle homogenized 1000 µl of reagent present in kit mixed samples and incubated for 10minutes at 25°C and absorbance was read at 500 nm of the standard and samples against reagent blanks within 60 minutes (Lopes et al., 1977, McGowan et al., 1983) and serum urea level was determined by taking one ml of working solution and 10µl of sample was taken in test tube against standard and dH 2 O (blank), left for 5 minutes at 25°C, finally absorbance was taken using spectrophotometer at 580nm. Body weight was determined by weight machine on every third day. "
ABSTRACT: This study was undertaken to investigate the effect of Garlic (Allium sativum) and Ginger (Zingiber officinale) aqueous extracts on serum glucose, cholesterol, urea levels and body weight in normal and alloxan-induced diabetic male rabbits. Before alloxan monohydrate (120mg/kg) introduction rabbits were left in fasting condition till twelve hours. After seven days of alloxan monohydrate induction diabetes mellitus was confirmed animals with fasting serum glucose level ≥ 250mg/dl and were considered as diabetes. In whole experimental work twelve male rabbits were selected divided in to four groups and each group contains three male rabbits 1 st and 2 nd group normal and diabetic control 3 rd to 4 th group containing male rabbits were treated with 1% garlic and ginger aqueous extract to check the comparison between them against serum glucose and their variable effects on serum cholesterol, serum urea and body weight. Blood serum was used to determine all selected biochemical parameters, on every third day. It was observed that 1% Garlic and ginger aqueous extract controlled 51% and 45.26% glucose, 35.29% and 21.95% cholesterol, 46.22% and 21.69% urea level and body weight gradually increased in alloxan induced diabetic male rabbits. It is observed in the present investigation that oral administration of garlic and ginger aqueous extracts are hypoglycemic agents but according to the results garlic is the best hypoglycemic agent, which also controlled cholesterol and urea level more than ginger in alloxan-induced diabetic male rabbitsPakistan Journal of Biotechnology 12/2013; 10(2).