Production of 2-phenylacetic acid and phenylacetaldehyde by oxidation of 2-phenylethanol with free immobilized cells of Acetobacter aceti
(Impact Factor: 2.52).
02/2004; 39(6):749-753. DOI: 10.1016/S0032-9592(03)00185-7
Acetobacter aceti MIM 2000/28 was employed for the oxidation of 2-phenyl-1-ethanol. Oxidation in aqueous systems gave phenylacetic acid with high yields, while the use of a two-liquid phase system (composed of water and isooctane) allowed for the production of the corresponding aldehyde. Free cells showed poor operational stability and were immobilized in calcium alginate; immobilized cells had specific activity, substrate tolerance and stability higher than that obtained with free cells. Simple fed-batch operation with immobilized cells in an air-lift reactor allowed for the production of 23 g l−1 phenylacetic acid in 9 days without foam formation.
Available from: Peter Gemeiner
- "f the encapsulated cells ( Figure 6 right ) was also investigated and a slightly decreased biotransforma - tion rate was observed . The cumulative concentra - tion of product ( Figure 7 ) after 7 days was 25 g / L . This is slightly higher than the product concentra - tion obtained after 9 - day cultivation in an air - lift reactor as described by Gandolfi et al . ( 2004 ) ."
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ABSTRACT: A high-performance biocatalyst in the form of encapsulated cells of Gluconobacter oxydans have been developed for production of phenylacetic acid (PAA) as a natural flavor component. Polyelectrolyte complex (PEC) capsules consisting of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl2, and NaCl were used for highly controlled and mild encapsulation of cells. Utilization of encapsulated G. oxydans cells was a significant improvement on existing data on operational stability of cells and cumulative product concentration during biocatalytic production of PAA from 2-phenylethanol. Concerning operational stability, encapsulated cells were active over 12 cycles with a high biotransformation rate, while free cells were inactive after 7 cycles of use. The biocatalytic properties of encapsulated G. oxydans were tested in a bubble column reactor over 7 days with a final cumulative product concentration of 25 g/L. High cell viability (90%) was observed within PEC capsules by confocal laser scanning microscopy, performed before and after repetitive PAA production in the bubble column reactor. The surface microstructure of fully hydrated capsules with and without G. oxydans cells was investigated and compared using an environmental scanning electron microscope.
Biocatalysis and Biotransformation 06/2015; 33(2). DOI:10.3109/10242422.2015.1053470 · 0.69 Impact Factor
28th Annual Symposium on Frequency Control. 1974; 02/1974
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ABSTRACT: A novel synthetic approach for the preparation of arylacetic acids via the reaction of aldehydes, N-(2,3,4,6-tetra-O-pivaloylated-d-glucopyranosyl)amine and trimethylsilylcyanide was developed, in which the N-(2,3,4,6-tetra-O-pivaloylated-d-glucopyranosyl)amine can be recycled conveniently and reused efficiently.
Tetrahedron 06/2005; 61(23):5671-5677. DOI:10.1016/j.tet.2005.03.095 · 2.64 Impact Factor
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