Article

C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice.

Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences No. 427, Harbin 150001, China.
The Veterinary Journal (impact factor: 2.24). 07/2012; DOI:10.1016/j.tvjl.2012.06.005
Source: PubMed

ABSTRACT Mycobacterium tuberculosis heat shock protein 70 (HSP70) and the peptide binding C-terminal portion of HSP70 (amino acids 359-610; HSP70c) exert an adjuvant effect when used in vaccines. To enhance the immunogenicity of a DNA vaccine against porcine circovirus type 2 (PCV2), recombinant plasmids encoding the PCV2 ORF2 (capsid) gene fused to full length hsp70 (pCA-TCH) or truncated C-terminal hsp70c (pCA-TCHc) were constructed. Immunisation of mice with pCA-TCHc induced higher serum immunoglobulin G antibody levels, stronger T helper 1 immune responses and lower PCV2 viral titres following challenge than immunisation with pCA-TCH or Cap plasmids only.

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Keywords

DNA vaccine
 
full length hsp70
 
lower PCV2 viral titres
 
Mycobacterium tuberculosis heat shock protein 70
 
pCA-TCHc
 
pCA-TCHc induced higher serum immunoglobulin G antibody levels
 
PCV2
 
PCV2 ORF2
 
stronger T helper 1 immune responses
 
truncated C-terminal hsp70c
 
vaccines
 

Fang Fu