Spectrophotometric and HPLC determination of secnidazole in pharmaceutical tablets
ABSTRACT Simple and accurate spectrophotometric and HPLC methods were developed for the determination of secnidazole in tablets dosage form. The first spectrophotometric method depends on the reduction of secnidazole molecule with zinc dust and hydrochloric acid followed by condensation with either p-dimethylaminobenzaldehyde or anisaldehyde to give colored chromogens having absorbance at 494 and 398 nm, respectively. The second method was based on the reaction of the drug with sodium nitroprusside in the presence or absence of hydroxylammonium hydrochloride. The formed colored chromogens were measured at 584 and 508 nm, respectively. The experimental conditions were optimized and Beer’s law was obeyed over the applicable concentration ranges. The application of HPLC procedures depended on using either a conventional or microbore reverse-phase (C18) column along with mobile phases consisting of water and methanol (30:70), at pH of 3.5. Both techniques were applied successfully for the analysis of secnidazole in tablets form. The results obtained from both procedures were statistically compared using the Student’s-t and F-variance ratio tests.
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ABSTRACT: Tax-free diesel fuel is intended for off-road uses such as agricultural operations, but illicit use of this fuel does occur and is a convenient way of evading payment of excise taxes. Current enforcement to prevent this practice involves visual inspection for the red azo dye added to the fuel to indicate its tax-free status. This approach, while very effective, has shortcomings such as the invasive nature of the tests and/or various deceptive tactics applied by tax evaders. A test designed to detect illicit dyed-fuel use by analyzing the vehicle exhaust would circumvent these shortcomings. This paper describes the development of a simple color spot test designed to detect the use of tax-free (i.e., dyed) fuel by analyzing the engine exhaust. Development efforts first investigated the combustion products of C.I. Solvent Red 164 (the azo dye formulation used in the United States to tag tax-free fuel). A variety of aryl amines were identified as characteristic molecular remnants that appear to survive combustion. A number of micro-analytical color tests specific for aryl amines were then investigated. One test that detected aryl amines by reacting with 4-(dimethylamino)benzaldehyde seemed to be particularly applicable and was used in a proof-of-principle experiment. The 4-(dimethylamino)benzaldehyde color spot test was able to clearly distinguish between engines that were burning regular fuel and those that were burning dyed diesel fuel. Further development will refine this color spot test to provide an easy-to-use field test.Talanta 10/2011; 86:148-56. · 3.50 Impact Factor
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ABSTRACT: Drug analysis is important in several phases of drug development, such as formulation, stability studies and quality control. The importance of reliable analytical methods for drug determination in a fast, inexpensive, sensitive and selective way is thus evident. Although there are countless works describing new analytical methods for determination of drugs that act against tropical diseases, a review organizing these works in a systematic and complete way is lacking. In this context, the objective of this review is to present the main advances in the development of analytical methods for de-termination of tropical disease drugs using electroanalytical, spectrophotometric and liquid chromatographic techniques.Current Pharmaceutical Analysis - CURR PHARM ANAL. 01/2009; 5(1).