Article

Preliminary investigation on antimicrobial and proteolytic property of the epidermal mucus secretion of marine stingrays

Center of Advanced Study in Marine Biology, Annamalai University, Portonovo, P.O. Box 608 502, Tamil Nadu, India
Asian Pacific Journal of Tropical Biomedicine 10/2011; DOI: 10.1016/S2221-1691(11)60162-7

ABSTRACT Objective
To determine the antibacterial, antifungal, minimum inhibitory concentration (MIC) and the protease activity of the stingray mucus Dasyatis sephen (D. sephen) and Himantura gerrardi (H. gerrardi).Methods
Antimicrobial activity of crude aqueous, acidic and organic mucus extract was evaluated by disc diffusion method against human pathogens, MIC of the active samples were determined by spectrophotometric method and the protease activity which is responsible for the antimicrobial activity was determined by using zymogram method.ResultsThe crude acidic extracts of both the species showed antibacterial activity against Salmonella typhi (S. typhi), Klebsiella pneumoniae, Streptococcus aureus, Escherichia coli (E. coli), Vibrio cholerae (V. cholerae) and the acidic extracts of both the species exhibit antifungal activity against all the tested pathogens. Remaining extracts didn't show any inhibitory activity. The acidic extracts of H. gerrardi is significantly active against S. typhi, E. coli, V. cholerae, Trichophyton mentagrophytes (T. mentagrophytes), Alternaria alternaria (A. alternaria), Trichophyton rubrum (T. rubrum), Candida tropicalis (C. tropicalis) at the minimum concentration of 16μ g/mL, but the acidic extract of D. sephen required 32μ g/mL of protein to inhibit S.typhi, E. coli, Aspergillus niger (A. niger), penicillium sp, T. mentagrophytes, A. alternaria. Both the D. sephen and H. gerrardi shows the proteolytic activity above the molecular mass of > 66 KDa. The characterization of protease class using inhibitors showed the presence of both serine and metallo protease in the the samples.Conclusions
Protease activity present in the sting ray mucus is one of the key factor responsible for the antimicrobial activity and the results proved the role of mucus in the innate immunity.

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