The effect of harpin protein on plant growth parameters, leaf chlorophyll, leaf colour and percentage rotten fruit of pepper plants inoculated with Botrytis cinerea

Department of Plant Protection, Uludag University, Boursa, Bursa, Turkey
Scientia Horticulturae (Impact Factor: 1.37). 06/2006; 109(2):107-112. DOI: 10.1016/j.scienta.2006.03.008


In this study, harpin protein was applied to the peppers (Capsicum annuum L. var. cvs. ‘Demre’, ‘Yalova Charleston’ and ‘Sari Sivri’) grown under natural conditions. These plants were subjected to artificial inoculation with Botrytis cinerea, which causes fruit spoilage in peppers. Changes in vegetative growth, total chlorophyll content in leaves, leaf colour and percentage of rotten fruits were determined after treatments. The number of leaves per plant value was quite low in all cultivars and the plant height value was low only in cv. ‘Sari Sivri’ treated with B. cinerea. Values obtained from vegetative growth parameters in the plants subjected to harpin protein + B. cinerea treatment were only higher than B. cinerea treatment. Leaf chlorophyll values exhibited significant decline in the plants subjected to B. cinerea treatment in all cultivars. However, the chlorophyll content in the plants subjected to harpin protein + B. cinerea treatment was low. The colour values obtained from leaves supported the chlorophyll findings. Fruit spoilage percentages were lower in the fruits picked from the plants of harpin protein + B. cinerea treatment compared with those picked from the plants only subjected to B. cinerea treatment.

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    • "A number of studies have reported the beneficial effect of harpin on plant growth in addition to the enhancement of disease resistance. For example, harpin treatments were found to affect leaf chlorophyll and plant growth in pepper (Akbudak and others 2006). Hpa1, a harpin protein produced by Xanthomonas oryzae, enhances plant growth and induces the expression of genes responsible for growth promotion (Chen and others 2008; Dong and others 2004; Liu and others 2006; Wu and others 2007). "
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    ABSTRACT: Harpin protein enhances plant disease resistance by activating PAMP-induced immunity. In this study, applications of harpin enhanced the efficiency of photosystem II, resulting in an increase in the fresh weights of Phalaenopsis orchids. A transcriptomic study of these harpin-treated Phalaenopsis orchids revealed that the application of harpin activated the expression of genes involved in nitric oxide (NO) generation, secondary metabolite production and plant responses, including responses to biotic, light stress, senescence, and hypoxia stimuli. Moreover, exposure to harpin induced the expression of a nitrate transporter and different members of the jasmonic acid resistance family. A proteomic study indicated that harpin applications increased protein accumulation in Phalaenopsis leaf tissues, including the accumulations of Cu/Zn superoxide dismutase (CSD2), pathogenesis-related protein 2, plasma membrane H+-ATPase, and protochlorophyllide oxidoreductase. In summary, this study demonstrates the effects of harpin on defense activation and growth promotion in Phalaenopsis orchids at both the physiological and molecular levels. Moreover, our study demonstrates that harpin modulates plant gene expression, most likely through its effects on reactive oxygen species (ROS), NO, and jasmonic acid signaling pathways.
    Journal of Plant Growth Regulation 12/2014; 33(4). DOI:10.1007/s00344-014-9425-1 · 2.24 Impact Factor
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    ABSTRACT: The objective of this work was to study the effects of Harpin, a bio-activator, and modified atmosphere packaging (MAP) on storage life and quality of cherry tomato (Lycopersicon esculentum Mill. cv. ‘Naomi’). Tomatoes grown in greenhouse were treated three times with Harpin. Fruits harvested at light red stage were stored in plastic film materials with different O2 and CO2 permeabilities and stored at 5- 6°C and 90±5% RH. Changes in the quality parameters were observed during the storage period at 7 day-intervals. Spoilage and maturity was accelerated in normal atmosphere (NA). Disorders were reduced with Harpin and low O2 and high CO2 during cold storage. Harpin combined with MAP produced better results than MAP alone. Therefore, Harpin and MAP treatments in combination proved effective in delaying ripening and maintaining fruit quality during storage. Harpin slowed the changes leading to quality loss in fruits for, 28 days of storage. Harpin treatment and 50 μm polyethylene (PE) packaging produced the best result.
    Acta horticulturae 06/2006; 712(712):237-243. DOI:10.17660/ActaHortic.2006.712.25
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    ABSTRACT: A 2 year field trial was conducted using established English oak (Quercus robur L.) to assess the efficacy of four commercially available systemic-inducing resistance (SIR) compounds (salicylic acid, potassium phosphite, harpin protein, be- taine) applied as a single therapeutic spray treatment against the foliar pathogen oak powdery mildew (Microsphaera alphitoides). In addition, a comparative evaluation of a conventional spray program (3 week spray intervals) used within the United Kingdom for powdery mildew control was conducted using the fungicide penconazole. The SIR-inducing compound containing betaine and a single spray treatment of penconazole had no significant influence on disease severity and specific activity of peroxidase and superoxide dismutase in both the 2005 and 2006 trials. Salicylic acid and potassium phosphite had no significant long-term effect on disease severity, although a short-term reduction in disease severity was recorded that was associated with enhanced leaf peroxidase and superoxide dismutase activity. A single therapeutic application of the SIR-inducing agent harpin protein signifi- cantly reduced disease severity of powdery mildew in the 2005 trial. No significant effects, however, were recorded in the 2006 trial. Only repeat spray applications of penconazole significantly reduced disease severity of oak powdery mildew in the 2005 and 2006 trials. The fungicide penconazole appears also to posses marginal SIR properties.
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