The effect of harpin protein on plant growth parameters, leaf chlorophyll, leaf colour and percentage rotten fruit of pepper plants inoculated with Botrytis cinerea
ABSTRACT In this study, harpin protein was applied to the peppers (Capsicum annuum L. var. cvs. ‘Demre’, ‘Yalova Charleston’ and ‘Sari Sivri’) grown under natural conditions. These plants were subjected to artificial inoculation with Botrytis cinerea, which causes fruit spoilage in peppers. Changes in vegetative growth, total chlorophyll content in leaves, leaf colour and percentage of rotten fruits were determined after treatments. The number of leaves per plant value was quite low in all cultivars and the plant height value was low only in cv. ‘Sari Sivri’ treated with B. cinerea. Values obtained from vegetative growth parameters in the plants subjected to harpin protein + B. cinerea treatment were only higher than B. cinerea treatment. Leaf chlorophyll values exhibited significant decline in the plants subjected to B. cinerea treatment in all cultivars. However, the chlorophyll content in the plants subjected to harpin protein + B. cinerea treatment was low. The colour values obtained from leaves supported the chlorophyll findings. Fruit spoilage percentages were lower in the fruits picked from the plants of harpin protein + B. cinerea treatment compared with those picked from the plants only subjected to B. cinerea treatment.
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ABSTRACT: Harpins can induce systemic acquired resistance (SAR) pathway on scores of non-host plant, provide protection against a range of pathogens. In this study, we demonstrated that applied recombinant HarpinZ Pseudomonas syringae pv. tomato (rHrpZ) on tobacco with three kinds of methods: infiltrating from micro-pore into leaf; injecting into petiole, and spraying on leaf, there is great difference in assimilation of protein because of the poor osmosis of tobacco leaves, and with multi-application of rHrpZ, the stimulation effect decreased. We prepared poly d,l-lactide-co-glycolide nanoparticles containing rHrpZ (rHrpZ PLGA NPs). To study the drug effect, we analyzed the change ratio of phenylalanine ammonia lyase (PAL) activity and PR-5dB gene expression after administration of rHrpZ and rHrpZ PLGA NPs on tobacco leaves. The results show that rHrpZ could elicit a rapid and transient increase in both PAL activity and PR-5dB expression, but the effect decreased after multi-application. While sprayed rHrpZ PLGA NPs on leaves, both the change ratio of PAL activity and PR-5dB expression were comparatively smooth and durable. Our study suggested that rHrpZ NPs could help protein enter leaf epidermis and cell wall, release rHrpZ in situ continuously, and enhance the bioavailability of rHrpZ.Journal of Biotechnology 09/2009; 143(4):296-301. · 3.18 Impact Factor
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ABSTRACT: A detached leaf bioassay was used to determine the influence of several film forming polymers and a conventional triazole fungicide on apple scab (Venturia inaequalis (Cooke) G. Wint.) development under laboratory in vitro conditions, supported by two field trials using established apple cv. Golden Delicious to further assess the efficacy of foliar applied film forming polymers as scab protectant compounds. All film forming polymers used in this investigation (Bond, Designer, Nu-Film P, Spray Gard, Moisturin, Companion PCT12) inhibited germination of conidia, subsequent formation of appressoria and reduced leaf scab severity using a detached leaf bioassay. Regardless of treatment, there were no obvious trends in the percentage of conidia with one to four appressoria 5 days after inoculation. The synthetic fungicide penconazole resulted in the greatest levels of germination inhibition, appressorium development and least leaf scab severity. Under field conditions, scab severity on leaves and fruit of apple cv. Golden Delicious treated with a film forming polymer (Bond, Spray Gard, Moisturin) was less than on untreated controls. However, greatest protection in both field trials was provided by the synthetic fungicide penconazole. Higher chlorophyll fluorescence Fv/Fm emissions in polymer and penconazole treated trees indicated less damage to the leaf photosynthetic system as a result of fungal invasion. In addition, higher SPAD values as measures of leaf chlorophyll content were recorded in polymer and penconazole treated trees. Application of a film forming polymer or penconazole resulted in a higher apple yield per tree at harvest in both the 2005 and 2006 field trials compared to untreated controls. Results suggest application of an appropriate film forming polymer may provide a useful addition to existing methods of apple scab management.Crop Protection - CROP PROT. 01/2009; 28(1):30-35.
- Gastroenterology 01/2011; 140(5). · 12.82 Impact Factor