Regeneration in Jatropha curcas: factors affecting the efficiency of in vitro regeneration. Ind Crops Prod

Industrial Crops and Products (Impact Factor: 2.84). 07/2011; 34(1):943-951. DOI: 10.1016/j.indcrop.2011.02.017


Factors influencing in vitro regeneration through direct shoot bud induction from hypocotyl explants of Jatropha curcas were studied in the present investigation. Regeneration in J. curcas was found to be genotype dependent and out of four toxic and one non-toxic genotype studied, non-toxic was least responsive. The best results irrespective of genotype were obtained on the medium containing 0.5 mg L−1 TDZ (Thidiazuron) and in vitro hypocotyl explants were observed to have higher regeneration efficiency as compared to ex vitro explant in both toxic and non-toxic genotypes. Adventitious shoot buds could be induced from the distal end of explants in all the genotypes. The number of shoot buds formed and not the number of explants responding to TDZ treatment were significantly affected by the position of the explant on the seedling axis. Explants from younger seedlings (≤15 days) were still juvenile and formed callus easily, whereas the regeneration response declined with increase in age of seedlings after 30 days. Transient reduction of Ca2+ concentrations to 0.22 g L−1 in the germination medium increased the number of responding explants.

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    • "eron la induc - ción de brotes mediante el uso de BAP en combinación con AIB . Existen diversos trabajos relacionados con la regeneración de Jatropha curcas L . desarrollados a tra - vés de la organogénesis directa , utilizando diferentes tipos de explantes tal es el caso de ( Sujatha y Mukta , 1996 ; Kumar y Reddy , 2010 ; Kumar et al . , 2010a ; Sharma et al . , 2011 , Kumar y Reddy , 2012 ) que a través de peciolos , cotiledones e hipocotilos lograron la regeneracion de la especie . Así mismo , Khurana - Kaul et al . ( 2010 ) ; Kumar et al . ( 2010c ) ; Misra et al . ( 2010a ) ; Kumar et al . ( 2011 ) , lograron la regeneración directa a partir de hojar ; mientras que , Wei et al . ( 2004 ) logró b"
    02/2015; DOI:10.18387/polibotanica.39.4
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    • "The majority of the reports for J. curcas have used plant growth regulators (PGRs) such as 6-benzylaminopurine (BAP), indoleacetic acid (IAA), indole-3-butyric acid (IBA), gibberellic acid (GA 3 ), kinetin (KN), and thidiazuron (TDZ) for culture generation using different explants, including nodal segments, shoot apices, hypocotyls, epicotyls, juvenile cotyledons, leaves, petioles, and stems with various regeneration efficiency (Sujatha et al. 2005; Jha et al. 2007; Shrivastava and Banerjee 2008; Khurana-Kaul et al. 2010; Singh et al. 2010; Varshney and Johnson 2010; Khemkladngoen et al. 2011; Sahoo et al. 2012; Zhang et al. 2013). The significant role of CuSO 4 and Ca 2+ in the shoot bud induction enhancement has been reported (Khurana-Kaul et al. 2010; Sharma et al. 2011). In our study, "
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    ABSTRACT: Jatropha curcas L. is attaining worldwide interest as an important biofuel crop. Experiments were conducted to improve the prevailing micropropagation technique as well as to develop a new ex vitro rooting method for J. curcas plant regeneration. Regeneration and ex vitro rooting efficiency was enhanced by augmenting the culture medium with abscisic acid (ABA). Different concentrations of 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) were tested for callus generation from both in vitro and in vivo explants (leaf and petiole) on Murashige and Skoog (MS) medium. The best regenerative callus was achieved on MS medium supplement-ed with BAP (4.44 μM) and IBA (2.45 μM) from in vitro-cultured petioles. Highest regeneration (91%) was achieved by culturing petiole callus on MS medium supplemented with BAP (8.88 μM), IBA (0.49 μM), and ABA (1.9 μM), whereas 61% regeneration was obtained from in vitro leaf callus. Shoot proliferation and elongation was achieved on BAP (2.22 μM) and IAA (8.56 μM) with 10–13 shoots per explants. Highest rooting (65%) was achieved from M1 shoots (BAP, IAA, and ABA) on MS medium supplemented with IBA (2.45 μM), naphthaleneacetic acid NAA (0.54 μM), and 0.02% activated charcoal. Ex vitro rooting of 1-mo-old M1 shoots obtained from the charcoal-containing medium resulted optimum rooting (>72%) when transferred to polybags containing ster-ile sand. The plantlets were successfully acclimatized in soil with more than 98% survival rate in the greenhouse.
    In Vitro Cellular & Developmental Biology - Plant 06/2014; 50(5). DOI:10.1007/s11627-014-9628-y/fulltext.html · 0.98 Impact Factor
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    • "Moreover, greater than 80% rooting was recorded for 2.0 mg L À1 IBA or IBA in various combinations with NAA and IAA. A similar root induction response was also reported by Sharma et al. [22] and Thiyagarajan and Venkatachalam [24] by using IBA, IAA, and NAA. Conversely, Philip et al. [25] obtained rooted plantlets from shoot tip cultures of P. nigrum by using 1 mg L À1 of NAA under normal photoperiod. "
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    ABSTRACT: In this study, a novel approach for in vitro regeneration of Piper nigrum L. has been applied in order to increase healthy biomass, phytochemicals and piperine production via reverse photoperiod (16hD/8hL). Leaf portions of the seed-derived plants were placed on an MS-medium fortified with different PGRs. Under 16hD/8hL, thidiazuron (TDZ; 4.0mg L(-1)) and BA (1.5mg L(-1)) was found to be the most effective (<90%) in callus induction. Two concentrations (1.5, 2.0mg L(-1)) of the IBA produced>80% shoots from callus cultures. Healthy shoots were transferred to rooting medium and higher percentage of rooting (<90%) was observed on IBA (1.5mg L(-1)). These in vitro tissues were subjected to amino acid analysis, spectrophotometry, and HPLC. ARG, SER, THR, and TYR were the most abundant components out of 17 amino acids. Higher amino acid production was observed under normal photoperiod (16hL/8hD) than under reverse photoperiod (16hD/8hL). The highest total phenolic content (TPC; 9.91mg/g-DW) and flavonoid content (7.38mg/g-DW) were observed in callus cultures incubated under 16hL/8hD than other tissues incubated under 16hD/8hL photoperiod. Higher DPPH and PoMo activities were observed in tissues incubated under 16hL/8hD photoperiod, while ABTS and Fe(2+) chelating activities were found higher in tissues incubated under reverse photoperiod. Significant quantities of piperine content were observed in all tissues except callus cultures. These results suggest that reverse photoperiod is a promising approach for callus induction, phytochemicals and piperine production for commercial applications.
    Comptes rendus biologies 01/2014; 337(1):19-28. DOI:10.1016/j.crvi.2013.10.011 · 0.98 Impact Factor
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