[Show abstract][Hide abstract] ABSTRACT: A vibrio-bacteriophage system isolated from San Francisco Bay mud is described. The vibrio lyses readily in distilled water and appears to be a new species. In contact with the phage it becomes lysogenic and forms large clumps. The phage presents no unique features.
The Journal of General Physiology 12/1954; 38(2):161-8. · 4.73 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A bacteriophage active against a marine Aeromonas sp. was isolated from surface sediments of the North Pacific Ocean at 825 m depth. The sensitive Aeromonas sp. grew between 0 and 33C but plaques were formed only between 0 and 23C with a maximum zone of lysis at 5 to 12C. The phage was rapidly inactivated at temperatures above 45C. The characteristic plaque formation occurred only on media made with sea-water but some lysis was obtained in distilled water media supplemented with 0.085 M NaCl and 0.05 M MgCl2. Phage replication occurred in cultures of Aeromonas sp. growing under applied hydrostatic pressures of up to 200 atmospheres. Electron microscopy revealed that the phage has an hexagonal head 530 in diameter, a thin tail 1200 in length and a terminal base plate 400 in diameter.
[Show abstract][Hide abstract] ABSTRACT: A rapid, simple and sensitive direct bacteriophage presence detection method for 500 ml drinking water samples has been developed. The method includes a glass device consisting of a jar containing the water sample and an immersible probe filled with solidified soft agar containing bacterial host cells. Host bacteria in logarithmic phase were added to the experimental volume and the probe was submerged. The entire device was incubated in a water bath at 36 degrees C. Plaques of somatic bacteriophage infecting Escherichia coli strain CN13, could be detected within 3 h. Male-specific bacteriophages infecting E. coli F+ amp were detected within 6 h. Bacteriophage infecting the anaerobe Bacteroides fragilis subsp. fragilis HSP40 were detected after 8 h. Application of this device and the associated technique, enabled a one-step detection of 1 pfu of E. coli or Bact. fragilis specific bacteriophage in 500 ml drinking water samples.
The Journal of applied bacteriology 05/1993; 74(4):490-6.
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