Article

Involvement of the skeletal Renin-Angiotensin system in age-related osteoporosis of ageing mice.

Center for Systems Biomedical Science, University of Shanghai for Science and Technology, Shanghai, PR China.
Bioscience Biotechnology and Biochemistry (impact factor: 1.28). 07/2012; 76(7):1367-71. pp.1367-71
Source: PubMed

ABSTRACT The local tissue-specific renin-angiotensin system (RAS) was identified. The aim of this study was to investigate the role of local bone RAS in the osteoporosis of aging mice. Twelve-month-old and two-month-old male mice were respectively assigned to the ageing and young groups. The tibias and femurs were collected for an analysis of histomorphology, bone mass, and gene and protein expression. H&E staining and micro-CT measurement showed a loss of the trabecular bone network and decrease of bone mineral density in the proximal tibial metaphysis of the aged mice. The PCR results indicated the significant up-regulation of renin and angiotensinogen (AGT) mRNA expression in both the tibia and femur of the ageing mice. Western blotting data showed that the tibial angiotensin II protein expression was significantly increased in the ageing group. The enhancement of renin and AGT expression in the bone tissue resulted in the increased production of angiotensin II which plays an important role in the pathology of age-related osteoporosis.

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Keywords

age-related osteoporosis
 
aged mice
 
ageing group
 
ageing mice
 
angiotensin II
 
angiotensinogen
 
bone mass
 
bone mineral density
 
increased production
 
local bone RAS
 
local tissue-specific renin-angiotensin system
 
micro-CT measurement
 
PCR results
 
protein expression
 
proximal tibial metaphysis
 
tibial angiotensin II protein expression
 
trabecular bone network
 
two-month-old male mice
 
Western blotting data
 
young groups
 

Sa-Sa Gu