A simple and rapid colorimetric method for the assay of erythrocyte and plasma glutathione reductase (GR) activity is described. The method is based on the colorimetric measurement of reduced glutathione (GSH) (1) produced when the enzyme is incubated with oxidised glutathione (GSSG) in the presence of either reduced nicotinamide adenine dinucleotide (NADH) or reduced nicotinamide adenine dinucleotide phosphate (NADPH). Results of investigations on the effects of substrate and coenzyme concentrations, pH, EDTA, sodium/potassium chloride, and time, on enzyme activity are presented. Erythrocyte and plasma NADH-GR and NADPH-GR activities in 100 healthy blood donors, and 85 cord blood samples and plasma NADH-GR and NADPH-GR levels in patients with various disease conditions are given.
[Show abstract][Hide abstract] ABSTRACT: An improved and optimized method for serum glutathione reductase is described. The reference range for normal subjects is 47-79 IU/1. The method is more sensitive than conventional enzyme tests in the detection of malignant disease. It was not raised more frequently in patients with clinical evidence of metastases than in those clinically free of such metastases, and it did not seem to correlate with prognosis among those patients who failed to survive six months from the time the analysis was first conducted.
[Show abstract][Hide abstract] ABSTRACT: Riboflavin nutritional status, based on erythrocyte glutathione reductase (EGR) determinations, and the absorption of the vitamin after 10-mg oral doses were assessed in a group of oral contraceptive users (group OC, n = 18) and in a group of nonusers (group NOC, n = 17). Before administration of test doses of riboflavin, mean EGR levels of 4.06 (group OC) and 4.44 (group NOC) mumoles glutathione reduced per minute per gram of hemoglobin were not significantly different. After oral administration of riboflavin, a significant increase in EGR levels was observed within each group although no significant differences were detected between groups. Mean EGR levels continued to be slightly lower for group OC compared to group NOC. Oral absorption of riboflavin based on urinary excretion results was not significantly different between the groups. These findings indicate OC use does not significantly affect riboflavin nutritional status in a subject population having adequate nutrition of moderate to high socioeconomic status. Significant reductions in EGR levels which have been previously reported for OC users appear to be associated with a low socioeconomic status.
American Journal of Clinical Nutrition 11/1979; 32(10):2047-51. · 6.77 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The activities of erythrocyte phosphoribosyl pyrophosphate (PRPP) synthetase and glutathione reductase (GTR) were studied in 26 patients with primary gout who were receiving no treatment or treatment with either allopurinol or azapropazone, and compared with the activity in a group of healthy controls. The activity of PRPP synthetase was significantly higher in the gout group and was not influenced by either drug. No significant difference in the activity of GTR was observed. The failure of either drug to suppress the increased activity of PRPP synthetase associated with gout is discussed.
Annals of the Rheumatic Diseases 12/1986; 45(11):941-4. DOI:10.1136/ard.45.11.941 · 10.38 Impact Factor
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