Calcium interaction with salinity-induced effects on growth and metabolism of soybean (Glycine max L.) cultivars. J Environ Biol

Journal of Environmental Biology (Impact Factor: 0.56). 09/2010; 31:795-801.


In the present work, hydroponic culture of JS-335 and Bragg cultivars of soybean (Glycine max) were raised to analyze changes in growth, reactive oxygen metabolism in terms of H2O2 content, lipid peroxidation (TBARS), free radical quenching systems (nonenzymatic and enzymatic antioxidants) and ion accumulation in different plant parts under NaCl and CaCl2 stress. Fifteen-day-old seedlings were treated with solutions of 25 mM (T1), 50 mM (T2) and 100 mM (T3) NaCI alone and in combination of 10 mM CaCl2 i.e., 25 mM+10 mM(T 4), 50 mM+ 10 mM (T5) and 100 mM+ 10 mM(T6). Observations recorded at 30 days after sowing displayed significant decreases in plant biomass, leaf water potential, leaf area, chlorophyll content and the contents of glutathione (GSH) and ascorbate (AsC) on application of NaCl alone. However, H2O2 content and lipid peroxidation (TBARS) in leaves were enhanced, consequently invoking the activities of SOD, APX, GR and CAT. Application of NaCl + CaCl2 alleviated adverse effects of NaCl stress. The Na+ and Cl- contents in different plant parts increased with NaCl as well as with NaCl+ CaCl2 treatments. The maximum accumulation occurred in roots, followed by the stem and the leaves. The K+ and Ca2+ contents decreased under NaCI stress; but NaCl + CaCl2 treatment reduced the extent of decrease caused by NaCl. Thus, calcium ameliorated the deleterious effects of NaCl stress and stimulated plant metabolism and growth.

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    • "These findings suggest that calcium can mitigate the sodium toxicity of plant. Many authors stated that exogenous calcium alleviates stress in Vigna radiata, Glycine max, Linum usitatissimum (Manivannan et al., 2007; Arshi et al., 2010; Khan et al., 2010). "
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    ABSTRACT: Nitric oxide (NO), a small diffusible, ubiquitous bioactive molecule, acts as prooxidant as well as antioxidant, and also regulates remarkable spectrum of plant cellular mechanisms. The present work was undertaken to investigate the role of nitric oxide donor sodium nitroprusside (SNP) and/or calcium chloride (CaCl2) in the tolerance of excised mustard leaves to salt stress. After 24 h, salt stressed leaves treated with SNP and/or CaCl2, showed an improvement in the activities of carbonic anhydrase (CA) and nitrate reductase (NR), and leaf chlorophyll (Chl) content, leaf relative water content (LRWC) and leaf ion concentration as compared with the leaves treated with NaCI only. Salinity stress caused a significant increase in H2O2 content and membrane damage which is witnessed by enhanced levels of thiobarbituric acid reactive substances (TBARS) and electrolyte leakage. By contrast, such increases were blocked by the application of 0.2 mM SNP and 10 mM CaCl2 to salt stressed leaves. Application of SNP and/or CaCl2 alleviated NaCl stress by enhancing the activities of antioxidative enzymes viz. superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione reductase (GR) and by enhancing proline (Pro) and glycinebetaine (GB) accumulation with a concomitant decrease in H2O2 content, TBARS and electrolyte leakage, which is manifested in the tolerance of plants to salinity stress. Moreover, application of SNP with CaCl2 was more effective to reduce the detrimental effects of NaCI stress on excised mustard leaves. In addition to this, ameliorating effect of SNP was not effective in presence of NO scavenger cPTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxidel. To put all these in a nut shell, the results advocate that SNP in association with CaCl2 plays a role in enhancing the tolerance of plants to salt stress by improving antioxidative defence system, osmolyte accumulation and ionic homeostasis.
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