Sperm DNA Fragmentation in Rams Vaccinated with Miloxan

Universidad Autónoma de Madrid, 28049, Madrid, Spain
The Open Veterinary Science Journal 02/2008; 2(1). DOI: 10.2174/1874318800802010007

ABSTRACT Sperm DNA fragmentation was analysed in 113 semen samples obtained from different rams over a period of one year. Semen samples were collected from: unvaccinated rams between January and June (Control group 1; CG1); vaccinated rams at least 70 days after vaccination between October and December (Control group 2; CG2); vaccinated rams 20 days after vaccination (Vaccinated group 1; VG1); and vaccinated rams 40 days after vaccination (Vaccinated group 2; VG2). Results show Miloxan, the vaccine of interest in this study, increased the percentage of sperm cells with fragmented DNA by 10-fold on average (from 6.5±7.9 to 63.4±24.2). However, the negative impact of vaccination on sperm DNA fragmentation appeared to reversible; near normal levels of sperm DNA fragmentation had been restored 40 days after vaccination (21.7±10.6). On the basis of these data, the use of semen samples from vaccinated animals should be avoided until at least one month after vaccination.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The quality of sperm DNA is very important in maintaining the reproductive potential of men. Sperm DNA is resistant to many types of insults that occur during its journey from the testis to time it reaches the oocyte for fertilization. During natural conception, a selection process occurs that allows only sperm with intact DNA to fertilize an oocyte. When assisted reproductive techniques (ART) are used, some or all of this selection process is bypassed. As a result, sperm with damaged DNA may fertilize an oocyte. Damage to sperm nuclear DNA negatively affects assisted and natural fertility. Increasingly, sperm DNA is being recognized as an independent measure of sperm quality that may have better diagnostic and prognostic capabilities than standard sperm parameters. This review summarizes the available evidence for the role of sperm DNA damage in assisted fertility. Two important facts are obvious from the available evidence: 1) men with spermatozoal DNA damage appears to have a decreased ability to father offspring, 2) spermatozoa with abnormal DNA can fertilize an oocyte, which may progress to a live birth. Infertile men who are planning to undergo ART procedures with their partner should have their sperm evaluated for DNA damage. The results of this evaluation may be used to counsel the couple about their chances for live birth and for genomic abnormalities in their offspring.
    Minerva ginecologica 07/2004; 56(3):235-45.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cigarette smoking is a serious health problem of our society. It is known that cigarette smoke is a cell mutagen and carcinogen, and that it may affect adversely male fertility. The possible detrimental effects on sperm cells are of great interest but the data available to support this statement are somewhat elusive. To approach this problem we examined conventional semen parameters, plasma membrane translocation of phosphatidylserine (PS) (annexin V/6-CFDA cell staining) and sperm DNA integrity (comet assay) in a group of healthy man smoking cigarettes on a regular basis. The results of the study were compared with the results of the same tests in healthy non-smoking donors. Significant difference in standard sperm parameters between the two groups was not found. Intensive expression of PS on the sperm plasma membrane surface (assayed by annexin V positive staining) was detected in the smokers group. There is a significant increase of population of apoptotic spermatozoa in ejaculates of smokers. Albeit DNA damages (high frequencies of double- and single- stranded DNA breaks) in spermatozoa of smokers are increased compared with non-smokers, but this difference is not statistically significant. Sperm DNA integrity of healthy smokers remains in the normal range, but a clear negative trend is observed, especially in respect of disturbance of plasma membrane phospholipid asymmetry.
    International Journal of Andrology 11/2004; 27(5):296-300. DOI:10.1111/j.1365-2605.2004.00486.x · 3.21 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The influence of critical telomeric attrition, a well-known trigger of apoptosis and cell arrest, on sperm DNA fragmentation was studied in late-generation knockout mice for Terc, the RNA component of telomerase, as a model of choice. Terc knockout mice had a sixfold mean increase in the percentage of sperm cells with fragmented DNA.
    Fertility and sterility 11/2005; 84(4):843-5. DOI:10.1016/j.fertnstert.2005.05.014 · 4.30 Impact Factor


1 Download
Available from