Article

Aplicaciones de los cerdos transgénicos en biomedicina y producción animal

01/2010; 106:30-45.

ABSTRACT Resumen Los animales transgénicos han supuesto un gran avance en el campo de la ciencia y de la salud. Los avances tecnológicos en este campo han permitido desarrollar una amplia variedad de cerdos trans-génicos con diversos objetivos y aplicaciones. En este documento hemos descrito y analizado las diver-sas aplicaciones que los cerdos transgénicos han tenido y tienen en el ámbito biomédico y en el agro-pecuario. Entre estas aplicaciones en el campo de la salud destacamos los modelos de enfermedad humana, la generación de productos biofarmaceuticos y el transplante de órganos de cerdos modifi-cados a humanos (xenotransplante). Por otra parte, la resistencia a las enfermedades, la mejora de los indices productivos y la reducción del efecto contaminante de la actividad ganadera son las principa-les aplicaciones de los cerdos transgénicos en producción animal. El uso de cerdos transgenicos está más desarrollado en el campo de la biomedicina que en el agropecuario, fundamentalmente por las limitaciones que existen actualmente en el consumo de productos alimentarios derivados de los orga-nismos modificados genéticamente (OMG's). Palabras clave: salud, modelos enfermedad, biofármacos, xenotransplantes, mejora productiva, con-taminación. Summary Applications of transgenic pigs in biomedicine and animal production Transgenic animals have been a great improvement for science and health. The lastest technological developments have lead to the generation of a broad variety of transgenic pigs with diverse aims and applications. In this manuscript, we describe and analyze the usage and applications of the transgenic pigs in biomedicine and agriculture. The applications of transgenic pigs in biomedicine included models for human diseases, production of bio-pharmacological substances and xenotransplantation. The agriculture applications of transgenic pigs are disease resistance, increases in the production and quality of animal products and pollution reduction. The use of transgenic pigs is higher in biomedici-ne than in agriculture. This is due to the severe restriction of consumption of food products derived from genetic modified organisms (GMO's).

0 Bookmarks
 · 
165 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Haemophilia replacement factors, both plasma-derived and recombinant, are in relatively short supply and are high-cost products. This has stymied the study and development of alternative methods of administration of haemophilia therapy even in the most economically advanced countries, owing to the large amounts of material needed because bioabsorption and bioavailability of haemophilic factors can be less than 10% when using non-intravenous routes of delivery. There is therefore a need to increase access to therapy worldwide by decreasing the cost and increasing the abundance so that therapy can be achieved through simplified, alternative delivery methods. Transgenic livestock have been used to produce haemophilic factors in milk. Only the pig mammary gland has been shown to carry out the post-translational processing necessary to enable both the biological activity and long circulation half-life needed for therapeutic glycoproteins. Furthermore, the large amounts of recombinant protein that can be produced from pig milk make feasible the use of alternative delivery methods such as oral, intratracheal, subcutaneous, and intramuscular administration.
    Haemophilia 11/2004; 10 Suppl 4:70-6. · 3.17 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The goal of this study was to determine whether the presence of the bovine alpha-lactalbumin transgene in first-lactation gilts enhances lactational performance and litter growth. Transgenic and sibling nontransgenic gilts were bred to nontransgenic boars. Litters were standardized to 10 piglets within 24 h of farrowing. Milk production was measured by the weigh-suckle-weigh method on d 3, 6, 9, and 12 of lactation. Bovine alpha-lactalbumin was present in the colostrum and milk of transgenic gilts throughout lactation. The expression of the transgene was associated with alterations in composition of mammary secretions, especially in early lactation. Lactose concentrations were greater (P < 0.05) in mammary secretions of transgenic gilts during the first 12 h postpartum compared with controls. In contrast, total solids concentration in mammary secretions from transgenic gilts were lower (P < 0.05) relative to controls during the first 6 h postpartum. Transgenic gilts produced more milk than controls on d 3, 6, and 9 of lactation (P < 0.01). By d 12, differences in milk production between transgenic and control sows were no longer different. Lactose intake by transgenic-reared litters was greater than lactose intake by control-reared litters on d 6 of lactation (P < 0.05). Total solids intake was significantly greater (P < 0.05) by transgenic-reared litters on d 3 and 6 compared to control-reared litters. The day x genotype interaction on litter weight gain after birth was highly significant (P = 0.011), with transgenic-reared litters gaining weight at a greater rate than control-reared piglets. Expression of the transgene was associated with increased milk production in lactating gilts and increased growth of transgenic-reared piglets. Increased lactose synthesis in response to the presence of the transgene may result in increased milk production in early lactation, leading to increased milk component intake by transgenic litters, and ultimately to increased growth of litters reared by first-parity transgenic gilts.
    Journal of Animal Science 05/2002; 80(4):1090-6. · 2.09 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We introduced a fusion gene of human albumin and enhanced green fluorescent protein (EGFP) into porcine oocytes using the sperm vector method, and produced a piglet that showed clear expression of GFP in the hooves and skin. PCR and Southern blotting analysis of genomic DNA extracted from the piglet's tissues, including the liver, showed that the tissues carried the transgene. RT-PCR analysis demonstrated that both the human albumin and EGFP genes were expressed in the tissues. The fact that human albumin gene was integrated and expressed in the liver of the transgenic pig opened a way for us to achieve our goal, which was the use of transgenic pigs for the bioartificial liver support system.
    Journal of Reproduction and Development 09/2005; 51(4):539-46. · 1.76 Impact Factor

Full-text (2 Sources)

View
434 Downloads
Available from
Jun 3, 2014