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Polymorphism of C3 complement component in sheep

Department of Animal Genetics, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria; Institute of Agricultural Science, Bulgaria; Department of Animal Genetics; Agrarian Faculty, Trakia University, Bulgaria
Trakia Journal of Sciences Trakia Journal of Sciences 01/2005; 3(3):30-36.

ABSTRACT This investigation aimed to determine whether the C3 complement component in sheep was of a polymorphic nature. The sheep included in this study were the milk type crossings as follows: 25 sheep and 6 rams -Stara Zagora × East-Friesian and (Stara Zagora × East-Friesian) × Blackhead Pleven breed; 10 male and 19 female lambs (their progeny) were also included in the study. The electrophoretic mobility and the phenotype expression of C3 complement component isovariants were expressed by one or two bands with differing electrophoretic mobility. The one that was moving slower was labelled with the letter S, whereas the faster one was labelled with the letter F. The homozygous phenotypes were marked with SS and FF, while the heterozygous with FS. Apart from these principal phenotypes, some others were observed, particularly in the heterozygous variant. They were the F5S, F7S and F10S phenotypes. They differed by the distance between the F and S bands. In the ordinary heterozygous phenotype, the F−S distance was 3 mm, whereas for the others they were 5, 7 and 10 mm, respectively. On the basis of familial analysis we concluded that the C3 bands are inherited in a co-dominant way.

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    ABSTRACT: This research included sheep and rams of the following three breeds: Trakia merino – 80 sheep and 9 rams; Ile-de-France – 107 sheep; Charollais – 107 sheep; and milk crossbreeds: Stara Zagora × East-Friesian, and (Stara Zagora × East-Friesian) × Blackhead Pleven breeds – 118 sheep and 14 rams. The genetic structure of the studied sheep populations was determined by the calculation of the relative frequency of the observed alleles of the gene coding for the C3 complement component (S, F, F1, S1), and the genotypes that they formed (SS, FF, FS, F7S, F5S, F10S, FF1, SS1, S1S1). The S allele had the highest frequency in the milk type, Ile-de-France and Trakia merino. The Charollais breed demonstrated the reverse phenomenon as it had the F allele with a higher frequency. In this breed, another allele, F1, was observed. Its frequency was low, yet it was present in the genetic structure of the studied population of Charollais sheep. In the sheep of the Trakia merino breed, we discovered a fourth allele – S1. It also had a very low frequency – 0.04. The four alleles described formed the nine genotypes established by us -SS, FF, FS, F7S, F5S, F10S, FF1, SS1, and S1S1. Their frequencies differed in the different breeds of sheep, yet most common was the homozygous genotype SS (milk type, Ile-de-France, and Trakia merino). Second was the heterozygous type FS that had the highest frequency in the Charollais breed. The heterozygous type F7S should be noted as it had rather high frequencies in two of the breeds (Ile-de-France – 0.33 and Trakia merino – 0.32).
    Trakia Journal of Sciences Trakia Journal of Sciences. 01/2005; 3(3):34-38.

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