Article

Influence of magnetic iron oxide nanoparticles on red blood cells and Caco-2 cells

Laboratory of Biophysics, Saarland University, 66123, Campus, Saarbruecken, Germany; UMR 7504, Institut de Physique et Chimie des Matériaux de Strasbourg, CNRS, Université de Strasbourg-ECPM, 67034, Strasbourg Cedex 2, France
Advances in Bioscience and Biotechnology 01/2010; 1:439-443.

ABSTRACT The interactions of two types of cells (red blood cells, Caco-2 cells) with magnetic iron oxide nanoparticles (non-grafted, citrate-grafted, dendrimer-grafted) of 11 nm in size have been investigated. We focused on two important physiological parameters of the cells, the intracellular pH and the intracellular Ca 2+ con-tent. The results show that the nanoparticles do not have a significant influence on the pH and Ca 2+ con-tent of Caco-2 cells. The Ca 2+ content of red blood cells is also not affected but the intracellular pH is slightly reduced.

0 Bookmarks
 · 
123 Views
  • Advanced Functional Materials 04/2009; 19(10):1553 - 1566. · 9.77 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study evaluated cellular uptake of polymeric nanoparticles by using Caco-2 cells, a human colon adenocarcinoma cell line, as an in vitro model with the aim to apply nanoparticles of biodegradable polymers for oral chemotherapy. The feasibility was demonstrated by showing the localization and quantification of the cell uptake of fluorescent polystyrene nanoparticles of standard size and poly(lactic-co-glycolic acid) (PLGA) nanoparticles coated with polyvinyl alcohol (PVA) or vitamin E TPGS. Coumarin-6 loaded PLGA nanoparticles were prepared by a modified solvent extraction/evaporation method and characterized by laser light scattering for size and size distribution, scanning electron microscopy (SEM) for surface morphology, zeta-potential for surface charge, and spectrofluorometry for fluorescent molecule release from the nanoparticles. The effects of particle size and particle surface coating on the cellular uptake of the nanoparticles were quantified by spectrofluorometric measurement. Cellular uptake of vitamin E TPGS-coated PLGA nanoparticles showed 1.4 folds higher than that of PVA-coated PLGA nanoparticles and 4-6 folds higher than that of nude polystyrene nanoparticles. Images of confocal laser scanning microscopy, cryo-SEM and transmission electron microscopy clearly evidenced the internalization of nanoparticles by the Caco-2 cells, showing that surface modification of PLGA nanoparticles with vitamin E TPGS notably improved the cellular uptake. It is highly feasible for nanoparticles of biodegradable polymers to be applied to promote oral chemotherapy.
    Biomaterials 06/2005; 26(15):2713-22. · 8.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The gastrointestinal uptake of micro- and nanoparticles has been the subject of recent efforts to develop effective carriers that enhance the oral uptake of drugs and vaccines. Here, we used correlative instrumental neutron activation analysis and electron microscopy to quantitatively and qualitatively study the gastrointestinal uptake and subsequent tissue/organ distribution of 4, 10, 28, and 58 nm diameter metallic colloidal gold particles following oral administration to mice. In our quantitative studies we found that colloidal gold uptake is dependent on particle size: smaller particles cross the gastrointestinal tract more readily. Electron microscopic studies showed that particle uptake occurred in the small intestine by persorption through single, degrading enterocytes in the process of being extruded from a villus. To our knowledge this is the first report, at the ultrastructural level, of gastrointestinal uptake of particulates by persorption through holes created by extruding enterocytes.
    Journal of Pharmaceutical Sciences 01/2002; 90(12):1927-36. · 3.13 Impact Factor

Full-text (2 Sources)

View
79 Downloads
Available from
Jun 4, 2014