Antioxidant isozymes activities in potato plants (Solanum tuberosum L.) under salt stress

University of Tehran, 1016-1104, ISSN; Seed and Plant Improvement Campus, Agricultural Biotechnology Research Institute of Iran, P.O. Box, 31535-1897, Karaj, Islamic Republic of Iran; Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Islamic Republic of Iran
Islamic Republic of Iran 01/2006; 17:225-230.

ABSTRACT To determine NaCl effects on growth and some antioxidant enzymes activity, the internode cuttings of four potato cultivars (Solanum tuberosum L.), Agria, Kennebec (relatively salt tolerant), Diamant and Ajax (relatively salt sensitive) were grown on callus inducing media amended with 0, 50, 100 and 150 mM NaCl at dark conditions. Callus growth of all cultivars was significantly reduced under salt stress. Total superoxide dismutase (SOD) activity decreased in all cultivars when calli were grown in the presence of NaCl. Peroxidase (POD), H 2 O 2 detoxifying enzyme activity increased under salt stress, but at higher NaCl levels the activity was reduced. On the other hand, SOD and POD isozyme profiles at 100 mM NaCl were different from that of the control. These differences were quantitative and were expressed more in terms of increased or decreased isozymes activities. In dark conditions, callus tissue underwent oxidative stress in the presence of NaCl. In these conditions, more than any other SOD isozymes, Mn-SOD seems to play a major role in the scavenging of superoxide radicals during NaCl stress. Therefore, the increased Mn-SOD activity in salt treated-calli could reflect sustained O 2 − production in the mitochondria. On the other hand, in spite of increased activities of Mn-SOD and POD, reactive oxygen species (ROS) had damaging effects on the plant cells due to the SOD reduced total activity. Generally, there was no difference between relatively salt sensitive and tolerant cultivars in response to NaCl stress.

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    ABSTRACT: Differential expression of antioxidant enzymes in various growth and differentiation stages has been documented in several plant species. We studied here, the difference in the levels of protein content and antioxidant enzymes activity at two stages of maturity, named young and mature in neem (Azadirachta indica A. Juss), pigeonpea (Cajanus cajan (L.) mill sp) and mulberry (Morus Alba L.) leaves. The results showed that detached neem and pigeonpea mature leaves possessed higher activities of catalase (CAT) and peroxidase (POD) and lower activities of polyphenol oxidase (PPO) and ascorbate peroxidase (APX) as compared with young leaves. However, glutathione reductase (GR) showed higher activity in mature leaves of neem, whereas no change in its activity was observed in pigeonpea. On the other hand, antioxidant enzymes in mulberry showed either positive (PPO) or negative (POD, GR, APX) correlation with the progression of leaf maturity. Apparently the trend of changes in antioxidant enzymes activity during leaf development is species-specific: their activity higher at mature stage in some plants and lower in others.
    Plant signaling & behavior 10/2012; 7(10).
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    10/2012: pages 59-88; , ISBN: 978-953-51-0789-7


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