Single spore isolation of fungi

Fungal Diversity 01/1999; 3.

ABSTRACT W.H. (1999). Single spore isolation of fungi. Fungal Diversity 3: 29-38. Methods to isolate fungi from single spores are outlined. These methods are specifically designed for mycological laboratories which are not necessarily well funded. Therefore, they involve a simple procedure, are relatively inexpensive, and most importantly effective. Furthermore, only basic equipment is required. By using these methods, most fungi, with the exception of those that do not germinate on artificial medium, can be isolated. Some approaches are suggested to prevent mite infestations and to reduce the risk of bacterial contamination.

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Available from: Wellcome Wai Hong Ho, Sep 03, 2014
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    • "C. canescens was recognized by its typical growth with cercosporin production (Daub, 1982) along with typical conidia and conidiophores (Ellis and Martin, 1882). In a few cases isolations were made from the infected tissues and culture was further purified by single spore isolation (Choi et al., 1999). Spore producing isolates of C. canescens were deposited in Microbial Type "
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    • "The inoculated plates of PDA were incubated for 7-9 days at room temperature 25 0± 2 0 C. The colonies of the fungus were obtained within 8 days after inoculation. The pure cultures of the isolates were obtained using single spore isolation method described by Choi et al. (1999). The isolates were incubated on 2% potato dextrose agar (PDA) medium. "
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    ABSTRACT: The Chestnut (Castanea sativa Mill.) population in India is confined to the northern part of the country, which is continuously destroyed by natural (diseases/pests) and anthropogenic disturbances. Chestnut diseases like cankers and blight are mainly caused by fungi. Attempts were made to isolate the important fungal pathogen of chestnut trees. We isolated fungal isolates from samples of infected chestnut trees, which are confirmed as a new species of the genus Cytospora, family Valsaceae, with unique morphological and molecular characters. The initial identification of the fungus was based on morphological characters, and later confirmed by molecular studies. The phylogeny of the fungus was determined by rDNA-based phylogenetic markers ITS (Internal Transcribed Spacers) with the help of phylogenetic tools and were used for molecular identification and differentiation of the fungus. Phylogenetic analysis of the unknown fungus showed isolates reside in a clade separate from other species of genus Cytospora. Cytospora castanae sp. nov., therefore, is a new species of the genus Cytospora, witnessed by its morphological and molecular characters.
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    • "The selection medium was supplemented with 100 mg/ml hygromycin B (Roche, USA) or 500 mg/ml G418 (Ameresco , USA) for the screening for hygromycin B–resistant (HR) or G418-resistant (GR) transformants, respectively. Single conidium of the transformant was isolated to ensure nuclear homogeneity (Choi et al. 1999). If lack of conidia precluded single-spore isolation, then transformants were transferred three to four times to select against heterokaryons (Lev et al. 1999). "
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    ABSTRACT: A fungal pathogen Cochliobolus carbonum (anamorph, Bipolaris zeicola) causes Northern Leaf Spot, leading to a ubiquitous and devastating foliar disease of corn in Yunnan Province, China. Asexual spores (conidia) play a major role in both epidemics and pathogenesis of Northern Leaf Spot; but the molecular mechanism of conidiation in C. carbonum has remained elusive. Here, using a map-based cloning strategy, we cloned a single dominant gene, designated as BZcon1 (for Bipolaris zeicola conidiation), which encodes a predicted unknown protein containing 402 amino acids, with two common conserved SANT/Myb domains in N-terminal. The BZcon1 knockout mutant completely lost the capability to produce conidiophores and conidia, but displayed no effect on both hyphal growth and sexual reproduction. The introduced BZcon1 gene fully complemented the BZcon1 null mutation, restoring the capability for sporulation. These data suggested that the BZcon1 gene is essential for the conidiation of C. carbonum.
    G3-Genes Genomes Genetics 06/2014; 4(8). DOI:10.1534/g3.114.012286 · 3.20 Impact Factor
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