Single spore isolation of fungi


ABSTRACT W.H. (1999). Single spore isolation of fungi. Fungal Diversity 3: 29-38. Methods to isolate fungi from single spores are outlined. These methods are specifically designed for mycological laboratories which are not necessarily well funded. Therefore, they involve a simple procedure, are relatively inexpensive, and most importantly effective. Furthermore, only basic equipment is required. By using these methods, most fungi, with the exception of those that do not germinate on artificial medium, can be isolated. Some approaches are suggested to prevent mite infestations and to reduce the risk of bacterial contamination.

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    ABSTRACT: tThe general phenylpropanoid metabolism generates an array of secondary metabolites and phenolicacids which imparts disease resistance in plants. Expression pattern of the key genes of phenylpropanoidpathway was studied at 0, 24 and 48 h after infection (h.a.i.) and phenolic acid profiling was carried outat 0, 24, 48 and 72 h.a.i. in the leaves of wilt infected and non- infected of both resistant and susceptiblegenotypes of castor. Expression analysis of PAL (phenylalanine ammonia lyase), C4H1 (cinnamate 4-hydroxylase 1) and C4H2 (cinnamate 4-hydroxylase 2) genes using RT-PCR with gene specific primersshowed appreciable increase in the expression of PAL and C4H-2 gene in resistant genotypes at 48 hinterval than 24 h interval compared to susceptible genotype. However, gene C4H-1 was down regulatedin susceptible genotypes after 24 and 48 h.a.i. while up regulated in resistant genotypes. Phenol profilingusing HPTLC showed the presence of three phenolic acids i.e. caffeic acid, ferulic acid and salicylic acidin non-infected and infected castor genotypes. Higher content of caffeic and ferulic acid was detected ininfected and non-infected resistant genotypes at 0, 24 and 48 h.a.i, whereas caffeic acid was not detectedin susceptible genotypes at 0 h.a.i. These results suggest the critical role of phenols in castor disease resistance. © 2013 Elsevier B.V. All rights reserved.
    Industrial Crops and Products 10/2013; 50:456-461. · 3.21 Impact Factor
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    ABSTRACT: The biodegradation potentials of soil mycobiota isolated from six auto mechanic workshops and a farmland in Benin City on flow station crude oil sludge was investigated. Serial dilution and pour plate methods were utilized in the isolation and enumeration of the fungal bioload of the soil samples. The heterotrophic fungal counts ranged from 0.2×103 cfu/g to 3.2×103 cfu/g .Twenty (20) fungal species were identified from the soil samples; Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Aspergillus versicolor, Emericella nidulans, Aspergillus tamarii, Aspergillus niger, Aspergillus sp., Moniliella sp., Pichia farinosa, Sporobolomyces sp., Candida sp., Rhodotorula sp., Curvularia sp., Mucor sp., Rhizopus stolonifer, Penicillium sp. , Penicillium sp.2, Penicillium italicum, and Penicillium chrysogenum. A. flavus and A. nidulans had the highest percentage prevalence (85.7%). Physicochemical analyses revealed that the soil samples were acidic (pH 5.81-6.40) and sandy (50.3%-64.8%). Turbidimeteric screening revealed that A. flavus, A. terrus, Aspergillus sp., Penicillium sp., consortium of yeasts and the filamentous fungal consortium were able to maximally utilize the sludge as the sole source of carbon and energy. The growth profile results obtained for A. flavus revealed a decrease in pH (6.34 – 5.06) and an increase in turbidity (38 FAU – 625 FAU) during the 20 day incubation period. Amongst the growth profile cultures, A. flavus caused the highest percentage reduction in the residual TPH (DRO) content of the inoculated sludge (96%). Soils within the premises of automobile workshops can serve as a source of hydrocarbonclastic fungi.
    Journal of microbiology, biotechnology and food sciences 08/2013; 3(1):19-25.
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    ABSTRACT: The MBEC(TM)-High Throughput Assay based on the Calgary Biofilm Device was used to produce and to characterize Pleurotus ostreatus biofilms. Hydroxyapatite coating of pegs was required to enable biofilm attachment; biofilm amounts and homogeneity of distribution were markedly improved upon removal of non-sessile biomass after 48 h from inoculation. Scanning electron microscopy showed surface-associated and multi-layered growth stabilized by the presence of an extracellular matrix (ECM). Biofilms had higher contents of total sugars and ECM than their free-floating counterparts. Tolerance to Cr(VI) in the former was about twice that of the latter as inferred by the respective inhibitory concentrations (48.4 vs 24.1 mM and 114.5 vs 61.0 mM in 4- and 7-d-old cultures, respectively). Biofilms also displayed superior olive-mill wastewater (OMW) treatment efficiency along 5 consecutive batches leading to chemical oxygen demand and total phenol removals higher than 50 and 90%, respectively. Laccase activity peaks in biofilm cultures grown on OMW were significantly higher than those in free-floating cultures.
    Biofouling 09/2013; · 3.40 Impact Factor

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