Single spore isolation of fungi


ABSTRACT W.H. (1999). Single spore isolation of fungi. Fungal Diversity 3: 29-38. Methods to isolate fungi from single spores are outlined. These methods are specifically designed for mycological laboratories which are not necessarily well funded. Therefore, they involve a simple procedure, are relatively inexpensive, and most importantly effective. Furthermore, only basic equipment is required. By using these methods, most fungi, with the exception of those that do not germinate on artificial medium, can be isolated. Some approaches are suggested to prevent mite infestations and to reduce the risk of bacterial contamination.

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    ABSTRACT: The Chestnut (Castanea sativa Mill.) population in India is confined to the northern part of the country, which is continuously destroyed by natural (diseases/pests) and anthropogenic disturbances. Chestnut diseases like cankers and blight are mainly caused by fungi. Attempts were made to isolate the important fungal pathogen of chestnut trees. We isolated fungal isolates from samples of infected chestnut trees, which are confirmed as a new species of the genus Cytospora, family Valsaceae, with unique morphological and molecular characters. The initial identification of the fungus was based on morphological characters, and later confirmed by molecular studies. The phylogeny of the fungus was determined by rDNA-based phylogenetic markers ITS (Internal Transcribed Spacers) with the help of phylogenetic tools and were used for molecular identification and differentiation of the fungus. Phylogenetic analysis of the unknown fungus showed isolates reside in a clade separate from other species of genus Cytospora. Cytospora castanae sp. nov., therefore, is a new species of the genus Cytospora, witnessed by its morphological and molecular characters.
    Mycosphere. 11/2014; 5(6):747–758.
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    ABSTRACT: KD 2013 – Monograph of Cercosporoid fungi from Thailand. Plant Pathology & Quarantine 3(2), 67–138, doi 10.5943/ppq/3/2/2 The diversity of cercosporoid fungi in northern Thailand is very high. Eighty-five cercosporoid species were found in northern Thailand including (i) 84 species of true cercosporoid fungi: Cercospora (34), Passalora (7), Pseudocercospora (42), Zasmidium (1); (ii) One morphological similar fungus. Three new species were established, namely Pseudocercospora christellae, P. cratevae and P. radermachericola, while 23 cercosporoid species represent new records for Thailand. In this study, 50 species are described in full descriptions and illustrations, and another 35 species are only listed additionally because they have been described in ―Monograph of Cercosporid from Laos‖ or have previously been recorded from Thailand. The data show that the diversity of cercosporoid fungi in northern Thailand is very high; Meeboon (2009) recorded 166 cercosporoid species from this region.
    Plant Pathology and Quarantine. 01/2013; 3:19-90.
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Sep 3, 2014