Article

Comparison of effectiveness of ISSR and RAPD markers in genetic characterization of seized marijuana (Cannabis sativa L.) in Turkey

Department of Animal Science, Faculty of Agriculture, Biometry-Genetics Unit, Selcuk University, Konya, Turkey; Department of Field Crops, Faculty of Agriculture, Selcuk University, Konya, Turkey
African journal of agricultural research (Impact Factor: 0.08). 12/2010; 5:2925-2933.

ABSTRACT This paper presents results from comparison of effectiveness of Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and ISSR and RAPD (ISSR-RAPD) markers together in characterization of Cannabis accessions. The plant material used was common accessions of psychoactive Cannabis samples (a total of 17 accessions), which were used in discriminating drug type Cannabis from hemp type Cannabis via ISSR and characterization of Cannabis samples via RAPD. Data were analyzed via cluster and principal coordinate analyses (PCoA). Discriminating powers of ISSR and RAPD markers on the seized Cannabis accessions were evaluated by utilising polymorphism information content, resolving power and marker index (MI). The PCoA of ISSR and ISSR-RAPD markers data produced similar results. Average resolving power and MI values of ISSR assay found to be slightly higher than those of RAPD assay. Consequently, ISSR markers would be a better choice compared to RAPD markers in characterization of Cannabis accessions. Key words: Cannabis sativa L., individualization of seized marijuana, inter simple sequence repeat (ISSR), randomly amplified polymorphic DNA (RAPD), principal coordinate analysis, cluster analysis.

1 Bookmark
 · 
312 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia. ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia. ISSR markers showed clear distinction among the species whereas RAPD markers showed segregation based on geographical location as well as species based. Key words: Garcinia, genetic diversity, inter-simple sequence repeats, randomly amplified polymorphic DNA,
    AFRICAN JOURNAL OF BIOTECHNOLOGY 11/2013; Vol. 12(46), pp. ,(ISSN 1684-5315 ©2013 Academic Journals):6443-6451. · 0.57 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We investigated informativeness and effectiveness of different marker types (ISSR, IRAP, REMAP, RGAP and LP-PCR that employ primers based on the conservative sequences of abiotic stress response genes) to study genetic diversity of Iris pumila L. By the number of amplicons per primer, number of polymorphic amplicons per primer and resolving power index (Rp), ISSR-markers were the most efficient followed by LP-PCR-markers. In order of decreasing value of indicators of genetic diversity “the percentage of polymorphic bands”, and “the average Jaccardś genetic distance between plants”, marker systems may be arranged as follows: ISSR > RAPD > LP-PC > RGAP ≈ IRAP. For ISSR-markers, the percentage of polymorphic bands was 1.3–1.7 times higher than for the others, and the average genetic distance was 1.2–1.3 times higher. Different marker systems were ranked by the value of Neiś gene diversity and the Shannonś index as follows: ISSR > RAPD ≈ LP-PCR > RGAP ≈ IRAP, with the highest and the lowest values differing 1.4 times. Genetic population structure was investigated with program Structure 2.3. The data of all marker systems suggest that all genomes under study belonged to one population. The PCoA and cluster analyses based on genetic distances showed distinctions in clustering generated from different markers data and summarized data, as well as the lack of strong clusters. Mantel test revealed significant positive correlation between the matrices of genetic distances generated by the data of almost all marker systems. The strongest correlation was found between RGAP- and IRAP-markers (r = 0.452, p = 0.01) and between RGAP and ISSR (r = 0.430, p = 0.01). ISSR, RAPD and LP-PCR proved to be more effective for the study of I. pumila genetic diversity, nevertheless, joint use of different marker systems will provide a more comprehensive assessment of variation in different genomic regions.
    Biologia 01/2013; 68(4):613-620. · 0.51 Impact Factor

Full-text (2 Sources)

View
148 Downloads
Available from
Jun 5, 2014